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Templates for DNA-templated Fe(3)O(4) nanoparticles.

机译:DNA模板化Fe(3)O(4)纳米粒子的模板。

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摘要

Two different strategies are reported that result in the site-specific placement of long DNA molecules, templated with Fe(3)O(4) nanoparticles, on SiO(x) surfaces. The strategies combine solution templating of inorganic nanoparticles onto biomolecules, molecular combing, the layer-by-layer method, and dip-pen nanolithography (DPN). In one methodology, the first step is to place and stretch DNA molecules via a DPN template containing features of poly(allylamine hydrochloride) (PAH). In the second step the elongated DNA is templated with Fe(3)O(4) nanoparticles allowing them to assemble onto the DNA molecules based on electrostatic interactions. In the second methodology, DPN templates composed of PSS patterns are used to comb in a site-specific manner DNA molecules that have been pre-tempated in solution with the Fe(3)O(4) nanoparticles. The templates generated by the two methods can serve as building blocks for the fabrication of future higher order assemblies.
机译:报告了两种不同的策略,导致长DNA分子在SiO(x)表面上的模板与Fe(3)O(4)纳米粒子模板化的特定位置。这些策略将无机纳米颗粒的溶液模板化到生物分子上,进行分子梳理,逐层方法和浸笔式纳米光刻(DPN)相结合。在一种方法中,第一步是通过包含聚烯丙胺盐酸盐(PAH)功能的DPN模板放置和拉伸DNA分子。在第二步中,将细长的DNA与Fe(3)O(4)纳米粒子进行模板化,使它们可以基于静电相互作用组装到DNA分子上。在第二种方法中,由PSS模式组成的DPN模板用于以位点特异性方式梳理已在溶液中与Fe(3)O(4)纳米粒子预先结合的DNA分子。这两种方法生成的模板可以用作构建将来的更高阶程序集的基础。

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