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首页> 外文期刊>Photosynthesis Research: An International Journal >Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b ( 6 ) f complex: a simple and effective assay for stability of the complex in detergent solutions
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Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b ( 6 ) f complex: a simple and effective assay for stability of the complex in detergent solutions

机译：过氧化氢诱导的细胞色素b（6）f配合物中的叶绿素a漂白：一种简单有效的测定洗涤剂溶液中配合物稳定性的方法

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摘要

The instability of cytochrome b ( 6 ) f complex in detergent solutions is a well-known problem that has been studied extensively, but without finding a satisfactory solution. One of the important reasons can be short of the useful method to verify whether the complex suspended in different detergent is in an intact state or not. In this article, a simple and effective assay for stability of the complex was proposed based on the investigation on the different effects of the two detergents, n-octyl-beta-D: -glucopyranoside (OG) and dodecyl-beta-D: -maltoside (DDM), on the properties of the complex. DDM stabilizes the complex preparation more effectively whereas OG denatures the interactions of the heme groups and pigment molecules with the protein environment, leading to the bleaching of chlorophyll a induced by addition of hydrogen peroxide. The assay of the use of hydrogen peroxide to characterize the complex by studying the bleaching of chlorophyll induced by hydrogen peroxide and the peroxidase activity of the complex was discussed. This simple method will probably be useful to study the stability of the complex.
机译：细胞色素b（6）f络合物在去污剂溶液中的不稳定性是一个众所周知的问题，已经广泛研究，但是没有找到令人满意的溶液。重要原因之一可能是缺少验证悬浮在不同洗涤剂中的复合物是否处于完整状态的有用方法。在本文中，基于对两种去污剂正辛基-β-D：-吡喃葡萄糖苷（OG）和十二烷基-β-D的不同作用的研究，提出了一种简单有效的复合物稳定性测定方法：麦芽糖苷（DDM），对复合物的性质。 DDM更有效地稳定了复合制剂，而OG使血红素基团和色素分子与蛋白质环境的相互作用变性，导致过氧化氢的添加导致叶绿素a漂白。通过研究过氧化氢诱导的叶绿素的漂白和复合物的过氧化物酶活性，探讨了使用过氧化氢表征复合物的方法。这种简单的方法可能对研究复合物的稳定性很有用。

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