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首页> 外文期刊>Biomaterials >Chitosan-RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering.
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Chitosan-RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering.

机译：壳聚糖-RGDSGGC共轭物作为用于肌肉骨骼组织工程的支架材料。

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In the present study, we have developed a novel and versatile method for the preparation of chitosan-peptide complex based on the selective reaction of chitosan with 2-iminothiolane. The new type of SH-chitosan derivative showed an excellent solubility to aqueous solution even in the alkaline conditions. This characteristic greatly facilitated further modification study of chitosan with a variety of bioactive substances. A synthetic peptide, RGDSGGC containing RGDS moiety that is known as one of the most important cell adhesive peptides, was readily coupled by disulfide bonds formation with sulfhydryl groups of SH-chitosan in the presence of dimethyl sulfoxide. Next, the effect of the introduction of RGDSGGC moiety to chitosan on cell adhesion and proliferation activity of chondrocytes and fibroblasts were evaluated. As a result, it was suggested that this polysaccharide-peptide conjugate exhibited excellent capacities for both cell adhesion and cell proliferation of chondrocytes and fibroblasts. Considering the growing importance of the biocompatible scaffolds in the recent tailored tissue engineering technique, these results indicate that the present strategy of 2-iminothiolane-based conjugation of polysaccharides with biologically active peptides will become a key and potential technology to develop desirable scaffold materials for the tissue regenerations.
机译：在本研究中，我们基于壳聚糖与2-亚氨基硫杂环戊烷的选择性反应，开发了一种新颖且用途广泛的壳聚糖-肽复合物的制备方法。新型SH-壳聚糖衍生物即使在碱性条件下也显示出对水溶液的优异溶解性。这一特性极大地促进了壳聚糖对多种生物活性物质的进一步修饰研究。在二甲亚砜的存在下，合成的肽，即含有RGDS部分的RGDSGGC，被称为最重要的细胞粘附肽之一，很容易通过二硫键的形成与SH-壳聚糖的巯基结合。接下来，评估了将RGDSGGC部分引入壳聚糖中对软骨细胞和成纤维细胞的细胞粘附和增殖活性的影响。结果表明，该多糖-肽缀合物对软骨细胞和成纤维细胞的细胞粘附和细胞增殖均显示出优异的能力。考虑到生物相容性支架在最近量身定制的组织工程技术中日益重要的意义，这些结果表明，基于2-亚氨基硫杂环戊烷的多糖与生物活性肽缀合的当前策略将成为关键的和潜在的技术，以开发理想的支架材料。组织再生。

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