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首页> 外文期刊>Biomaterials >A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/periodontal ligament complex
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A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/periodontal ligament complex

机译：双相支架设计与细胞片技术相结合，可同时再生牙槽骨/牙周膜韧带

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摘要

This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum.
机译：这项研究描述了双相支架的设计，该支架由融合沉积建模支架（骨腔）和电纺膜（牙周腔）组成，用于牙周再生。为了同时实现牙槽骨和牙周膜的再生，通过结合成骨细胞在骨腔中的培养和在电纺膜上放置多个牙周膜（PDL）细胞片来实施基于细胞的策略。体外数据显示，培养21天后，成骨细胞在骨腔中形成矿化的基质，并且收集PDL细胞片不会诱导明显的细胞死亡。将细胞接种的双相支架置于牙本质块上，并在无胸腺大鼠皮下模型中植入8周。通过μCT，免疫组织化学和组织学分析支架。在成骨细胞中播种后，在骨腔中获得了更强烈的ALP染色，证实了μCT结果表明这些支架的矿化密度更高。通过H＆E和Azan染色观察，薄的矿化牙骨质样组织沉积在包含多个PDL细胞片的支架的牙本质表面上。与不使用细胞片时不附着相比，这些支架还表现出更好的附着在牙本质表面的能力。另外，免疫组织化学显示CEMP1蛋白在与牙本质的界面处存在。这些结果表明，多个PDL细胞片和双相支架的组合允许同时递送肺泡骨，牙周膜和牙骨质的体内再生所必需的细胞。

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