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首页> 外文期刊>Physical chemistry chemical physics: PCCP >Revealing the base pair stepping dynamics of nucleic acid motor proteins with optical traps
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Revealing the base pair stepping dynamics of nucleic acid motor proteins with optical traps

机译：用光阱揭示核酸运动蛋白的碱基对步进动力学

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摘要

Nearly all aspects of nucleic acid metabolism involve motor proteins. This diverse group of enzymes, which includes DNA and RNA polymerases, the ribosome, helicases, and other translocases, converts chemical energy in the form of bond hydrolysis into concerted motion along nucleic acid filaments. The direct observation of this motion at its fundamental distance scale of one base pair has required the development of new ultrasensitive techniques. Recent advances in optical traps have now made these length scales, once the exclusive realm of crystallographic techniques, accessible. Several new studies using optical traps have revealed for the first time how motor proteins translocate along their substrates in a stepwise fashion. Though these techniques have only begun to be applied to biological problems, the unprecedented access into nucleic acid motor protein movement has already provided important insights into their mechanism. In this perspective, we review these advances and offer our view on the future of this exciting development.
机译：核酸代谢的几乎所有方面都涉及运动蛋白。这种多样化的酶包括DNA和RNA聚合酶，核糖体，解旋酶和其他易位酶，将化学能以键水解的形式转化为沿着核酸细丝的一致运动。在一个碱基对的基本距离尺度上直接观察该运动需要开发新的超灵敏技术。一旦获得了晶体学的专有领域，光阱的最新进展就使这些长度标尺成为可能。使用光阱的几项新研究首次揭示了运动蛋白如何逐步地沿其底物转运。尽管这些技术才刚刚开始应用于生物学问题，但对核酸运动蛋白运动的前所未有的访问已经提供了对其机理的重要见解。从这个角度来看，我们回顾了这些进展，并对这一令人振奋的发展的未来提供了看法。

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