...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Phycological Research >Novel means for variety breeding and sporeling production in the brown seaweed Undaria pinnatifida (Phaeophyceae): Crossing female gametophytes from parthenosporophytes with male gametophyte clones
【24h】

Novel means for variety breeding and sporeling production in the brown seaweed Undaria pinnatifida (Phaeophyceae): Crossing female gametophytes from parthenosporophytes with male gametophyte clones

机译：在褐海藻Undaria pinnatifida（Phaeophyceae）中进行品种育种和孢子生产的新方法：将单性单性植物的雌配子体与雄配子体克隆杂交

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The unialgal haploid gametophyte clones are frequently used for variety breeding and sporeling production in the brown seaweed Undaria pinnatifida because a single crossing of a pair of the selected male and female gametophyte clones can generate sporophytic offspring with identical genotype and phenotype. As the seeds to be sprayed on the collectors, the detachment rate of the filamentous gametophytes is high in comparison to the seeded spores. In this investigation, we report the use of parthenogenesis to achieve the same goal in variety selection and sporeling production but with higher efficiency. The selected female unialgal gametophyte clone (Code: 06-8-1F) was induced to produce parthenosporophytes. These sporophytes were grown up in a controlled system and used to release zoospores. All zoospores generated into female gametophytes. These female gametophytes were allowed to go through parthenogenesis for the second year to confirm the applicability of this means. In the third year, the zoospores released from the parthenosporophytes were seeded on collectors over summer in female gametophyte form. In the early autumn, a selected male unialgal gametophyte clone (code: 5#F1-2-5M) was used to cross the seeded female gametophytes on the collectors. When the sporelings reached a mean length of 780m, they were transplanted to open sea on longlines for growing up. At harvest, the average length, width and wet weight of the adult sporophytes were 211cm, 48.8cm and 373g, respectively. The sporophytic blades were uniformly smooth without wrinkles on both sides of the midrib, indicating top quality of the products. Amplified fragment length polymorphism analyses confirmed the identical genotypes of sporophytic offspring. These results suggested that this novel variety breeding and sporeling production method could serve as an efficient alternative to the traditional breeding technique for U.pinnatifida and possibly other commercial kelps that have identical life cycles.
机译：单倍体单倍体配子体克隆经常用于褐海藻裙带菜（Undaria pinnatifida）的品种育种和孢子生产，因为一对选定雌雄配子体克隆的单次交配可产生具有相同基因型和表型的孢子体后代。由于将种子喷在收集器上，因此与种子孢子相比，丝状配子体的分离率高。在这项调查中，我们报告了使用孤雌生殖在品种选择和孢子生产中达到相同的目标，但效率更高。诱导选定的雌性单唾液酸配子体克隆（代码：06-8-1F）产生单性孢子体。这些孢子体在受控系统中生长，并用于释放游动孢子。所有游动孢子均生成雌配子体。这些雌配子体被允许进行第二年的孤雌生殖，以确认这种方法的适用性。第三年，从单性孢子体释放的游动孢子在夏季以雌配子体的形式播种在收集器上。在初秋，一个选定的雄性单配子配子体克隆（代码：5＃F1-2-5M）被用来与收集器上的种子雌配子体杂交。当孢子的平均长度达到780m时，它们就被移植到了延绳钓的远海中长大。在收获时，成年孢子体的平均长度，宽度和湿重分别为211cm，48.8cm和373g。孢子状叶片均匀光滑，中脉两侧均无皱纹，表明产品质量最高。扩增的片段长度多态性分析证实了孢子体后代的相同基因型。这些结果表明，这种新颖的品种育种和孢子生产方法可以作为传统的育种技术用于pinnatifida和可能具有相同生命周期的其他商业海藻的有效替代方法。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号