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首页> 外文期刊>Phycological Research >Intracellular nitrite accumulation: The cause of growth inhibition of Microcystis aeruginosa exposure to high nitrite level
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Intracellular nitrite accumulation: The cause of growth inhibition of Microcystis aeruginosa exposure to high nitrite level

机译:细胞内亚硝酸盐积累:铜绿微囊藻暴露于高亚硝酸盐水平时生长抑制的原因

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摘要

The aim of the present study is to test the role of intracellular nitrite in external nitrite suppressing algal growth. We examined the growth of Microcystis aeruginosa at different nitrite levels under high nitrate conditions and without nitrate conditions. There were higher intracellular nitrite and lower P-m(chla), R-d (chla), (chl), maximum cell density and specific growth rate in high nitrate group than nitrate absence group at 5mg NO2--N L-1. At 10 and 15mg NO2--N L-1, P-m(chla), R-d (chla), (chl), maximum cell densities and specific growth rates in the high nitrate group became higher than those of the nitrate absence group, while a lower intracellular nitrite in the high nitrate group than nitrate absence group was observed. In addition, the intracellular nitrite and the growth of M.aeruginosa in the high nitrate group did not change from 5 to 10mg NO2--N L-1. In the nitrite uptake experiment, with nitrite concentration increasing from 5 to 15mg NO2--N L-1, maximum nitrite uptake rate of alga increased, and half-saturation constant of alga decreased. These results indicate that external nitrite inhibited algal growth through stimulating intracellular nitrite rise, which resulted from overexpression of nitrite transporter.
机译:本研究的目的是测试细胞内亚硝酸盐在外部亚硝酸盐抑制藻类生长中的作用。我们研究了在高硝酸盐条件下和无硝酸盐条件下不同亚硝酸盐水平下铜绿微囊藻的生长。 5mg NO2--N L-1时,高硝酸盐组的细胞内亚硝酸盐含量较高,而P-m(chla),R-d(chla),(chl),最大细胞密度和比生长速率较低。在10和15mg NO2--N L-1,Pm(chla),Rd(chla),(chl)时,高硝酸盐组的最大细胞密度和比生长速率变得高于硝酸盐缺乏组。高硝酸盐组的细胞内亚硝酸盐含量低于无硝酸盐组。此外,高硝酸盐基团中的细胞内亚硝酸盐和铜绿假单胞菌的生长从5mg改变为10mg NO2--N L-1。在亚硝酸盐吸收实验中,随着亚硝酸盐浓度从5mg NO2--N L-1增加到15mg,最大藻类的亚硝酸盐吸收率增加,藻类的半饱和常数减小。这些结果表明外部亚硝酸盐通过刺激细胞内亚硝酸盐的上升而抑制了藻类的生长,这是由于亚硝酸盐转运蛋白的过表达引起的。

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