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首页> 外文期刊>Phycological Research >Artificial leaf aids analysis of chlorophyll fluorescence and P700 absorbance in studies involving microalgae
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Artificial leaf aids analysis of chlorophyll fluorescence and P700 absorbance in studies involving microalgae

机译：在涉及微藻的研究中人工叶助剂分析叶绿素荧光和P700吸收

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Measuring chlorophyll fluorescence and P700 absorbance has been widely used to study photosynthesis in both terrestrial plants and algae. However, in order to apply these measurement techniques to study microalgae, a concentrated suspension of algae, which is usually prepared by centrifugation, is required. In this study, instead of using centrifugation, we concentrated microalgae on a nitrocellulose membrane using filtration to create an artificial leaf' before analysis. Overall, we were able to generate values of the appropriate photosynthetic parameters that were comparable to those obtained when chlorophyll fluorescence and P700 absorbance were measured following centrifugation. There were no statistically significant differences (P>0.05) between the artificial leaf method and the traditional cuvette method for determining chlorophyll fluorescence or P700 absorbance at appropriate chlorophyll concentrations. We were also able to reduce background noise by using a filter membrane as a carrier. Therefore, an artificial leaf has the potential to be a valuable tool for phycologists interested in studying microalgal photosynthesis by enabling them to eliminate tedious centrifugation steps. In addition, fluorometers commonly used for studying the leaves of higher plants will also be suitable for studying microalgae.
机译：测量叶绿素荧光和P700吸光度已广泛用于研究陆地植物和藻类的光合作用。然而，为了将这些测量技术应用于微藻研究，需要通常通过离心制备的藻类浓缩悬浮液。在这项研究中，我们没有进行离心分离，而是在分析之前通过过滤将微藻类浓缩在硝酸纤维素膜上，以创建人造叶片。总体而言，我们能够生成与在离心后测量叶绿素荧光和P700吸光度时获得的光合作用参数相当的值。在适当的叶绿素浓度下，人工叶法和传统比色杯法测定叶绿素荧光或P700吸光度之间无统计学差异（P> 0.05）。通过使用滤膜作为载体，我们还能够减少背景噪音。因此，对于有兴趣研究微藻光合作用的植物学家来说，人造叶有可能成为有价值的工具，因为它们可以消除繁琐的离心步骤。此外，通常用于研究高等植物叶片的荧光计也将适用于研究微藻。

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