...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Physica status solidi, B. Basic research >Long-term adsorption of fetal bovine serum on H/O-terminated diamond studied in situ by atomic force microscopy
【24h】

Long-term adsorption of fetal bovine serum on H/O-terminated diamond studied in situ by atomic force microscopy

机译：原子力显微镜原位研究胎牛血清在H / O端接钻石上的长期吸附

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigate adhesion of fetal bovine serum (FBS) on diamond surfaces which have alternating H/O-terminated surface patterns of 30mm width prepared by hydrogen and oxygen plasma. The samples are immersed into 15% FBS in McCoy’s 5A supporting medium and characterized by in situ atomic force microscopy (AFM) during 6 days of adsorption. Within the first day, 2–4 nm primary layer is formed on both H-/O-terminated surfaces. After 6 days, we observe 17±5nm FBS layer on O-terminated surface and 35±5 nm layer on H-terminated surface. Adhesion of the primary and secondary layer is weaker on H-terminated surface. We present a model of FBS protein layers on H-/O-terminated diamond and we discuss implications for a preferential cell growth on O-terminated diamond. We also show that the preferential cell growth is not affected by the long-term FBS pre-adsorption.
机译：我们研究了胎牛血清（FBS）在钻石表面的粘附性，该钻石表面具有通过氢和氧等离子体制备的30mm宽的交替H / O端接的表面图案。将样品浸入McCoy的5A支持介质中的15％FBS中，并在吸附的6天中进行原位原子力显微镜（AFM）表征。在第一天之内，在两个H- / O端接的表面上形成了2-4 nm的第一层。 6天后，我们在O端表面观察到17±5nm FBS层，在H端表面观察到35±5 nm FBS层。在H端接的表面上，第一层和第二层的附着力较弱。我们提出了H- / O端钻石上FBS蛋白层的模型，并讨论了O端钻石上优先细胞生长的意义。我们还表明，优先的细胞生长不受长期FBS预吸附的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号