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首页> 外文期刊>Phytoparasitica >Effects of soil amendments combined with solarization on the soil microbial community in strawberry cultivation using quantitative real-time PCR
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Effects of soil amendments combined with solarization on the soil microbial community in strawberry cultivation using quantitative real-time PCR

机译：实时荧光定量PCR结合土壤改良剂和日晒对草莓栽培土壤微生物群落的影响

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Experiments were conducted in commercial fields of strawberry-growing areas of the Aydin Province of Turkey during two cropping seasons: 2010-2011 and 2011-2012. Each year, eight separate treatments were used: (1) untreated control (C), (2) solarization alone (S), (3) solarization + broccoli (SBr), (4) solarization + fava bean (SFB), (5) solarization + dry olive-mill wastewater (SDOMW), (6) solarization + rice hulls (SRH), (7) solarization + sulfur powder (SSu), and (8) solarization + vermicast (SVe). DNA was extracted from bulk soil samples before and after solarization. The populations of the major taxonomic groups of bacteria and soil-borne fungal pathogens of strawberry were quantitatively calculated by quantitative real-time PCR (qPCR) with specific primer pairs using standard curves. The marketable fruit yield was recorded in the experimental plots. After the 6-week solarization period, there were significant reductions for total bacteria and for alpha-Proteobacteria in all of the experimental plots, except for SDOMW. However, the abundance of beta-Proteobacteria significantly increased in all of the experimental plots (except for C and SFB in 2011). Significant increases in Firmicutes and Actinobacteria were also recorded in all of the treated plots. The highest significant increase was noticed with SDOMW treatments for total bacteria, alpha-Proteobacteria, beta-Proteobacteria, Firmicutes and Actinobacteria in both years. The target level of Verticillium spp. Rhizoctonia solani and Fusarium oxysporum decreased significantly in all of the treated plots after solarization in both years. The reductions of M. phaseolina were significant in S, SVe, and SSu and were the highest in SDOMW in 2011; these reductions were significant in S, SSu and SDOMW in 2012. The abundance of Trichoderma spp. decreased insignificantly in all of the experimental plots. The highest significant yields were obtained from the combination of SDOMW, SRH and SSu compared to solarization alone in both seasons.
机译：在两个种植季节：2010-2011年和2011-2012年，在土耳其艾登省草莓种植区的商业区域进行了试验。每年使用八种单独的处理方法：（1）未经处理的对照（C），（2）仅日晒（S），（3）日晒+西兰花（SBr），（4）日晒+蚕豆（SFB），（5 ）日光化+干橄榄磨坊废水（SDOMW），（6）日光化+稻壳（SRH），（7）日光化+硫磺粉（SSu）和（8）日光化+ mic粉（SVe）。在日晒前后，从大块土壤样品中提取DNA。草莓的细菌和土壤传播的真菌病原体的主要分类群的种群是通过使用标准曲线的特定引物对进行实时定量PCR（qPCR）进行定量计算的。在试验区记录了可销售的水果产量。经过6周的日晒期后，除SDOMW以外，所有实验区的细菌总数和α-变形杆菌均显着减少。但是，在所有实验区中，β-变形杆菌的丰度都显着增加（2011年C和SFB除外）。在所有处理过的地块中，菌丝和放线菌也显着增加。在这两年中，使用SDOMW处理的总细菌，α-变形细菌，β-变形细菌，硬毛和放线杆菌的增幅最高。黄萎病菌的目标水平。在这两年中，所有处理过的地块中，茄根枯萎病菌和尖孢镰刀菌均显着下降。 2011年，S，SVe和SSu的菜豆分枝杆菌减少幅度最大，SDOMW的减少幅度最高。这些减少在2012年对S，SSu和SDOMW而言是显着的。木霉属物种的丰富。在所有实验区中的下降幅度均很小。与两个季节中单独的日晒相比，从SDOMW，SRH和SSu的组合中获得最高的单产。

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