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首页> 外文期刊>Biomaterials >Improved tissue-engineered bone regeneration by endothelial cell mediated vascularization.
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Improved tissue-engineered bone regeneration by endothelial cell mediated vascularization.

机译：通过内皮细胞介导的血管形成改善组织工程化的骨再生。

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Natural bone growth greatly depends on the precedent vascular network that supplies oxygen and essential nutrients and removes metabolites. Likewise, it is crucial for tissue-engineered bone to establish a vascular network that temporally precedes new bone formation, and spatially originates from within the graft. In order to recapitulate physiological skeletal development, we have developed a complex bone graft to repair rat bone defects. We have demonstrated that endothelial cells and osteoblasts (identified by cell morphology, quantification of specific marker antigens, calcium deposition and capillary-like growth) were able to differentiate and expand from donor rat bone marrow mononuclear cell populations. The biocompatibilities of poly-epsilon-caprolactone (PCL)-hydroxyapatite (HA) composites used for graft fabrication were evaluated at different component ratios to identify the optimal and support of cellular viability and functions for endothelial cells and osteoblasts. Using point-injection and low-pressure techniques, seeded endothelial cells and osteoblasts were able to assemble into microvascular networks and form bony matrix in grafts. The exogenous origination of these cells and their contribution to the vascularization and osteogenesis was confirmed using sex-mismatch implantation and Y chromosome tracking. By pre-seeding with endothelial cells, the resulting vascularization was able to promote osteogenesis, prevent ischemic necrosis and improve the mechanical properties in engineered bone tissue. Taken together, the results indicated that the integration of complex cell populations with composite scaffold materials provided an effective technique to improve osteogenesis in engineered bone graft. These findings suggest that hybrid grafts have great potential for clinical use to treat large bone defects.
机译：骨骼的自然生长在很大程度上取决于先例的血管网络，该网络提供氧气和必需的营养素并清除代谢产物。同样，对于组织工程骨骼而言，建立在新骨骼形成之前暂时且在空间上源自移植物内部的血管网络至关重要。为了概括生理性骨骼发育，我们开发了一种复杂的骨移植物来修复大鼠骨骼缺损。我们已经证明，内皮细胞和成骨细胞（通过细胞形态，特定标记抗原的定量，钙沉积和毛细管样生长来鉴定）能够从供体大鼠骨髓单核细胞群中分化和扩增。在不同组分比例下评估了用于移植物制造的聚ε-己内酯（PCL）-羟基磷灰石（HA）复合材料的生物相容性，以确定对内皮细胞和成骨细胞的细胞生存力和功能的最佳和支持。使用点注射和低压技术，播种的内皮细胞和成骨细胞能够组装成微血管网络，并在移植物中形成骨基质。这些细胞的外源性起源及其对血管形成和成骨的作用已通过性别不匹配植入和Y染色体追踪得到证实。通过与内皮细胞预播种，所得的血管形成能够促进成骨，预防缺血性坏死并改善工程骨组织的机械性能。两者合计，结果表明复杂的细胞群体与复合支架材料的整合提供了一种有效的技术来改善工程骨移植的成骨性。这些发现表明，混合移植物具有巨大的临床应用潜力，可用于治疗大型骨缺损。

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