...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomaterials >The influence of laminin-derived peptides conjugated to Lys-capped PLLA on neonatal mouse cerebellum C17.2 stem cells.
【24h】

The influence of laminin-derived peptides conjugated to Lys-capped PLLA on neonatal mouse cerebellum C17.2 stem cells.

机译：与赖氨酸封端的PLLA偶联的层粘连蛋白衍生肽对新生小鼠小脑C17.2干细胞的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Chemical guiding cues are being exploited to stimulate neuron adhesion and neurite outgrowth. In this study, an amino-functioned PLLA, lysine-capped PLLA [K-(CH(2))(n)-PLLA (n=2, 5, 8)], was synthesized with different length of linking spaces between lysine molecule and PLLA backbone. Drop-cast films were fabricated from K-(CH(2))(n)-PLLA/PLLA blends (10/90, w/w) and amino groups were detected on the surfaces of the resultant films. More amine groups were detected on the surface and the hydrophilicity of the films was obviously improved by annealing the films in water. The representative atomic force microscopy (AFM) images indicated that incorporation of lysine-capped PLLA into PLLA matrix increased the roughness of the films and resulted in a phase separation with distinct two nano-domains which may correspond to the hydrophilic and hydrophobic domains. Furthermore, the laminin-derived peptides, CYIGSR (Cys-Tyr-Ile-Gly-Ser-Arg) and CSIKVAV (Cys-Ser-Ile-Lys-Val-Ala-Val), were jointly tethered to the amine groups of lysine-capped PLLA by a linking reagent sulfo-succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (Sulfo-SMCC). The neonatal mouse cerebellum C17.2 stem cells were seeded on the peptides-grafted K-(CH(2))(n)-PLLA/PLLA (n=2, 5, 8) films and pure PLLA films were used as controls. Improved viability and longer neurites were obtained on the peptide-grafted films than PLLA film over the cultivation period, especially for K-(CH(2))(5)-PLLA/PLLA, which had the highest peptide density of 0.28+/-0.03 microg/cm(2). This study highlights the potential of using the lysine-capped PLLA with laminin-derived peptides for promoting nerve regeneration.
机译：化学指导被用来刺激神经元粘附和神经突生长。在这项研究中，合成了具有氨基功能的PLLA，赖氨酸封端的PLLA [K-（CH（2））（n）-PLLA（n = 2，5，8）]，赖氨酸分子之间的连接空间长度不同和PLLA骨干。由K-（CH（2））（n）-PLLA / PLLA混合物（10/90，w / w）制成的流延薄膜，并在所得薄膜的表面上检测到氨基。通过在水中退火，可以在表面检测到更多的胺基，并且明显改善了薄膜的亲水性。代表性的原子力显微镜（AFM）图像表明，将赖氨酸封端的PLLA掺入PLLA基质中会增加薄膜的粗糙度，并导致相分离并出现两个明显的纳米域，这两个纳米域可能对应于亲水域和疏水域。此外，层粘连蛋白衍生肽CYIGSR（Cys-Tyr-Ile-Gly-Ser-Arg）和CSIKVAV（Cys-Ser-Ile-Lys-Val-Ala-Val）共同拴系在赖氨酸-的胺基上通过连接试剂磺基琥珀酰亚胺基4-（N-马来酰亚胺基甲基）环己烷-1-甲酸酯（Sulfo-SMCC）覆盖PLLA。将新生小鼠小脑C17.2干细胞播种在肽移植的K-（CH（2））（n）-PLLA / PLLA（n = 2、5、8）膜上，并将纯PLLA膜用作对照。在培养期间，与PLLA膜相比，在肽移植膜上获得了更高的生存力和更长的神经突，特别是对于K-（CH（2））（5）-PLLA / PLLA，其最高肽密度为0.28 +/- 0.03微克/厘米（2）。这项研究强调了将赖氨酸加帽的PLLA和层粘连蛋白衍生的肽用于促进神经再生的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号