Mixed peptide-chitosan membranes to mimic the biological activities of a multifunctional laminin alpha1 chain LG4 module.

Hozumi K; Yamagata N; Otagiri D; Fujimori C; Kikkawa Y; Kadoya Y; Nomizu M

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Mixed peptide-chitosan membranes to mimic the biological activities of a multifunctional laminin alpha1 chain LG4 module.

机译：混合的肽-壳聚糖膜可模拟多功能层粘连蛋白α1链LG4模块的生物活性。

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Laminin alpha1 chain LG4 module is multifunctional and interacts with syndecans and integrin alpha2beta1 via AG73 (RKRLQVQLSIRT) and EF-I (DYATLQLQEGRLHFMFDLG) sites, respectively. Here, we conjugated the AG73 and EF1zz (ATLQLQEGRLHFXFDLGKGR, X: Nle) peptides on a chitosan membrane in various ratios to develop an LG4 mimic biomaterial. The AG73-chitosan membrane promoted strong cell attachment with membrane ruffling and the EF1zz-chitosan membrane promoted integrin-mediated cell adhesion with well-organized actin stress fibers. When AG73 and EF1zz were conjugated on a chitosan membrane with 1:9 molar ratio, the mixed peptide-chitosan membrane promoted the strong cell attachment and neurite outgrowth similar to that on the recombinant LG4 protein. Well-organized actin stress fibers and vinculin accumulated focal contacts were observed in the cells attached on the AG73:EF1zz (molar ratio=1:9)-chitosan membrane. These results suggest that the mixed peptide-chitosan membrane interacts with both syndecans and integrin alpha2beta1 and mimics the cell adhesion of a multifunctional LG4 protein. The mixed peptide-chitosan approach has potential as a multifunctional biomaterial for cell and tissue engineering.

机译：层粘连蛋白α1链LG4模块具有多功能性，并分别通过AG73（RKRLQVQLSIRT）和EF-1（DYATLQLQEGRLHFMFDLG）的位点与Syndecans和整联蛋白alpha2beta1相互作用。在这里，我们将壳聚糖膜上的AG73和EF1zz（ATLQLQEGRLHFXFDLGKGR，X：Nle）肽偶联在一起，以开发出LG4模拟生物材料。 AG73-壳聚糖膜通过膜起皱促进了牢固的细胞附着，而EF1zz-壳聚糖膜则通过组织良好的肌动蛋白应激纤维促进了整合素介导的细胞粘附。当AG73和EF1zz以1：9的摩尔比结合在壳聚糖膜上时，混合的肽-壳聚糖膜促进了与重组LG4蛋白相似的牢固的细胞附着和神经突生长。在附着于AG73：EF1zz（摩尔比= 1：9）-壳聚糖膜上的细胞中观察到组织良好的肌动蛋白应力纤维和纽蛋白积累的焦点接触。这些结果表明，混合的肽-壳聚糖膜与双癸聚糖和整联蛋白alpha2beta1相互作用，并模仿多功能LG4蛋白的细胞粘附。混合肽-壳聚糖方法作为细胞和组织工程的多功能生物材料具有潜力。

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来源
《Biomaterials》 |2009年第8期|共8页
作者
Hozumi K; Yamagata N; Otagiri D; Fujimori C; Kikkawa Y; Kadoya Y; Nomizu M;
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正文语种 eng
中图分类医用一般科学;
关键词
poliglusam; Membranes; Peptides; Cells; laminin alpha1; 膜; 肽类; 细胞;

机译：poliglusam;Membranes;Peptides;Cells;laminin alpha1;膜;肽类;细胞;

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专利

1. Mixed peptide-chitosan membranes to mimic the biological activities of a multifunctional laminin alpha1 chain LG4 module. [J] . Hozumi K, Yamagata N, Otagiri D, Biomaterials . 2009,第8期

机译：混合的肽-壳聚糖膜可模拟多功能层粘连蛋白α1链LG4模块的生物活性。
2. Cyclic peptide analysis of the biologically active loop region in the laminin alpha3 chain LG4 module demonstrates the importance of peptide conformation on biological activity. [J] . Kato-Takagaki K, Suzuki N, Yokoyama F, Biochemistry . 2007,第7期

机译：层粘连蛋白α3链LG4模块中生物活性环区域的循环肽分析证明了肽构象对生物活性的重要性。
3. Cyclic peptides from the loop region of the laminin alpha 4 chain LG4 module show enhanced biological activity over linear peptides [J] . Yokoyama F, Suzuki N, Haruki M, Biochemistry . 2004,第42期

机译：来自层粘连蛋白α4链LG4模块环状区域的环肽显示出比线性肽更高的生物活性
4. Biological Activities of Loop Regions in the Laminin Alpha Chain G Domains [C] . Fumihiko Katagiri, Toshihiro Hara, Yuji Yamada Japanese peptide symposium . 2010

机译：椎体蛋白α链G域的循环区域的生物学活性
5. New methodologies for biological membrane mimics, electron-deficient assemblies, and cross-coupling of C-O based electrophiles. [D] . Leowanawat, Pawaret. 2013

机译：用于生物膜模拟，电子缺陷组装和基于C-O的亲电试剂的交叉偶联的新方法。
6. Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin sulfatides alpha-dystroglycan and several extracellular matrix proteins. [O] . J F Talts, Z Andac, W Göhring, 1999

机译：层粘连蛋白α1和α2链的G结构域和Perlecan与肝素硫化物α-dystroglycan和几种细胞外基质蛋白的结合。
7. Localization of the Laminin α4 Chain in the Skin and Identification of a Heparin-Dependent Cell Adhesion Site Within the Laminin α4 Chain C-Terminal LG4 Module [O] . Matsuura Hiroshi, Momota Yutaka, Murata Kaoru, 2004

机译：层粘连蛋白α4链在皮肤中的定位和层粘连蛋白α4链C端LG4模块内肝素依赖性细胞粘附位点的鉴定

Mixed peptide-chitosan membranes to mimic the biological activities of a multifunctional laminin alpha1 chain LG4 module.

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