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首页> 外文期刊>Phytopathologia Mediterranea >Fomitiporia mediterranea infecting citrus trees in Greece
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Fomitiporia mediterranea infecting citrus trees in Greece

机译:Fomitiporia内部感染希腊的柑橘树

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摘要

In recent years a serious disease of citrus (the orange cv. Washington navel, lemon and the common mandarin grafted on sour orange rootstocks) has been observed in southern Greek orchards. Affected trees decline, their leaves become yellow and fall early, and shoots and twigs die as the damage expands towards the trunk. Cross-sections of the trunks and large branches reveal a light-colored rot in the center, which is surrounded by brown hard necrotic wood. Symptoms start from pruned areas and spreadto the rootstock wood, and then resemble esca of grapevine. From the white rotted areas, a fungus was isolated on PDA that formed cream-yellow to light-brown colonies with dense aerial mycelium. Fungal fruit-bodies formed abundantly on the trunks of diseased trees. The fungus was identified as Fomitiporia mediterranea by both traditional and molecular methods. Pathogenicity tests were performed by artificially inoculating orange, mandarin, lemon and sour orange trees with the fungus. Control holes werefilled with two PDA plugs. Branches inoculated with the isolates from infected citrus showed wood discoloration that extended up to 20 cm above and 20 cm below the infection hole. The fungus was re-isolated from the discolored parts of the wood. Inoculations with isolates from grapevine and kiwi produced wood discoloration only 3-4 mm around the holes.
机译:近年来,在希腊南部的果园里发现了严重的柑橘病(橙色种,华盛顿肚脐,柠檬和嫁接在酸性橙色砧木上的普通话)。受影响的树木下降,叶子变黄并早落,随着伤害向树干延伸,芽和嫩枝死亡。树干和大树枝的横截面显示出中心的浅色腐烂,周围是褐色的硬质坏死木材。症状从修剪的区域开始,蔓延到砧木,然后类似于葡萄的丝瓜。从白色腐烂的区域,在PDA上分离出一种真菌,该真菌形成奶油黄色至浅棕色菌落,并带有密集的气生菌丝体。病果树的树干上大量形成真菌果体。通过传统方法和分子方法,该真菌均被鉴定为地中海中的Fmitiporia mediterranea。通过用真菌人工接种橙树,普通话,柠檬和酸橙树来进行致病性测试。控制孔中装有两个PDA插头。接种了来自受感染柑橘的分离株的分支显示出木材变色,延伸到感染孔上方20 cm和下方20 cm。从木材的变色部分重新分离出真菌。接种葡萄和猕猴桃的分离株后,孔周围仅变色了3-4毫米。

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