UV—VIS AND FLUORESCENCE STUDY ON PROTEINENRICHED SIMULATED BODY FLUID

E. LASZLOFFI; K. MAGYARI; V.SIMON

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UV—VIS AND FLUORESCENCE STUDY ON PROTEINENRICHED SIMULATED BODY FLUID

机译：富含蛋白的模拟体流体的UV-VIS和荧光研究

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UV-vis and fluorescence analyses were carried out to investigatethe spectroscopic changes induced by increasing concentration of bovine serumalbumin in a solution of simulated body fluid, prepared according to Kokuboprotocol. In this study are presented the results obtained from as prepared solutions.The linear increase of absorbance in UV-vis spectral region can be used to evaluate theprotein concentration in solution. The fluorescence intensity dependence on BSAconcentration has an inflection point at 1.41 mg/ml BSA in SBF, denoting adiminished fluorescence quantum yield for higher BSA concentration.

机译：进行了UV-vis和荧光分析，以研究根据Kokuboprotocol制备的模拟体液溶液中牛血清白蛋白浓度增加引起的光谱变化。在本研究中介绍了从制备好的溶液中获得的结果。紫外可见光谱区域中吸光度的线性增加可用于评估溶液中的蛋白质浓度。荧光强度对BSA浓度的依赖性在SBF中的BSA的拐点为1.41 mg / ml，表明较高BSA浓度的荧光量子产率降低。

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《Studia Universitatis Babes-Bolyai. Series Physica》 |2009年第1期|共8页
作者
E. LASZLOFFI; K. MAGYARI; V.SIMON;
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正文语种 eng
中图分类物理学;
关键词
Bovine serum albumin; simulated body fluid; fluorescence; UV-visspectroscopy;

机译：牛血清白蛋白;模拟体液;荧光;紫外可见光谱;

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1. UV—VIS AND FLUORESCENCE STUDY ON PROTEINENRICHED SIMULATED BODY FLUID [J] . E. LASZLOFFI, K. MAGYARI, V.SIMON Studia Universitatis Babes-Bolyai. Series Physica . 2009,第1期

机译：富含蛋白的模拟体流体的UV-VIS和荧光研究
2. MODIFICATION OF A TUNABLE UV-VISIBLE CAPILLARY ELECTROPHORESIS DETECTOR FOR SIMULTANEOUS ABSORBANCE AND FLUORESCENCE DETECTION - PROFILING OF BODY FLUIDS FOR DRUGS AND ENDOGENOUS COMPOUNDS [J] . Caslavska J., Thormann W., Gassmann E. Journal of chromatography, A: Including electrophoresis and other separation methods . 1995,第1期

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3. Contrary Effects of UV-Irradiation on In Vitro Apatite-Forming Ability of TiO2 Layer in Simulated Body Fluid [J] . K. Uetsuki, Y. Shirosaki, S. Hayakawa, Bioceramics Development and Applications . 2011,第1期

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4. Short Review: Effect of UV Light on Precipitation of Hydroxyapatite on TiO_2 in Simulated Body Fluid [C] . N. H. Mohd Idrus, H. Z. Abdullah International Conference on Mechanical and Manufacturing Engineering . 2013

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5. Biocorrosion studies of surface modified bioimplant material in simulated body fluids. [D] . Zaveri, Nikita A. 2008

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6. In Vitro Studies Evaluating Leaching of Mercury from Mine Waste Calcine Using Simulated Human Body Fluids [O] . John E. Gray, Geoffrey S. Plumlee, Suzette A. Morman, -1

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7. In Vitro Studies Evaluating Leaching of Mercury from Mine Waste Calcine Using Simulated Human Body Fluids [O] . Gray, John E., Plumlee, Geoffrey S., Morman, Suzette A., 2010

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UV—VIS AND FLUORESCENCE STUDY ON PROTEINENRICHED SIMULATED BODY FLUID

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