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首页> 外文期刊>Biomaterials >The influence of donor and hypoxic conditions on the assembly of cartilage matrix by osteoarthritic human articular chondrocytes on Hyalograft matrices.
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The influence of donor and hypoxic conditions on the assembly of cartilage matrix by osteoarthritic human articular chondrocytes on Hyalograft matrices.

机译：供体和低氧条件对透明质酸基质中骨关节炎人关节软骨细胞组装软骨基质的影响。

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Resurfacing of cartilage defects using cell-seeded, biomaterial grafts is a promising approach for articular cartilage repair and in this study we investigated the ability of human chondrocytes from osteoarthritic joints to generate cartilage tissue under standard conditions in cultured over 21 days on Hyalograft matrices under normoxic (20% O(2)) and hypoxic (5% O(2)) conditions. The results showed that constructs were more chondrogenic when cultured under hypoxic conditions, which resulted in greater production of sulphated glycosaminoglycan and collagen type II within the constructs and the cells expressed higher levels of genes encoding cartilage matrix proteins and chondrocyte transcription factors. However, there were very wide differences in the chondrogenic potential amongst donors as the weight ratio of total sulphated glycosaminoglycan to DNA in constructs varied from above 200 to below 10. These results establish that the generation of cartilage from human OA chondrocytes on biodegradable supports is favoured in lowered oxygen, but that under standard conditions, even at low passage, there is a large variation in the chondrogenic potential amongst chondrocytes from different donors. Further analysis of this variation suggested that the gene expression ratio of COL2A1/COL1A1 mRNA in the chondrocytes in monolayer culture may predict their subsequent performance in forming cartilage matrix on the Hyalograft scaffold.
机译：使用带细胞的生物材料移植物修复软骨缺损是关节软骨修复的一种有前途的方法，在这项研究中，我们研究了在正常条件下在透明质酸透明质酸基质上培养超过21天的标准条件下，来自骨关节炎关节的人软骨细胞产生软骨组织的能力。 （20％O（2））和低氧（5％O（2））条件。结果表明，在低氧条件下培养时，构建体具有更大的成软骨性，这导致构建体中硫酸糖胺聚糖和II型胶原的产生更多，并且细胞表达更高水平的编码软骨基质蛋白和软骨细胞转录因子的基因。但是，由于构建物中总硫酸化糖胺聚糖与DNA的重量比从200到低于10，供体之间的软骨形成潜力存在很大差异。这些结果表明，人OA软骨细胞在可生物降解支持物上的软骨生成是有利的在降低的氧气中，但是在标准条件下，即使在低通量的情况下，来自不同供体的软骨细胞之间的软骨生成潜能也存在很大差异。对该变异的进一步分析表明，单层培养的软骨细胞中COL2A1 / COL1A1 mRNA的基因表达比例可能预示了其随后在Hyalograft支架上形成软骨基质的性能。

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