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首页> 外文期刊>Phytochemistry >Benzoquinone, the substance essential for antibacterial activity in aqueous extracts from succulent young shoots of the pear Pyrus spp.
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Benzoquinone, the substance essential for antibacterial activity in aqueous extracts from succulent young shoots of the pear Pyrus spp.

机译：苯并醌，对梨梨多汁多汁幼芽的水提取物中的抗菌活性必不可少的物质。

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摘要

Aqueous extracts of the tissue of succulent young shoots of the pear Pyrus spp. exhibited strong antibacterial activity against the bacterium Erwinia amylovora bv. 4. This activity was investigated quantitatively by a newly developed bioassay method. It was found that the activity changed with the age of the tissue. Extracts of the youngest leaves and stems from the shoot tops showed the strongest activity, and the activity decreased with age of the leaves and stems. The activity also changed with increase in time after preparation of the extract, increasing rapidly in the first hour after preparation, reaching a maximum at about 4 h, and then decreasing slowly. The substance essential for the antibacterial activity was isolated from the extract by steam distillation in vacuo and through charcoal powder column chromatography. It was identified as benzoquinone (2,5-cyclohexadiene-1,4-dione) by NMR-spectra, mass spectra and HPLC analysis. The phenolic metabolism from arbutin to hydroquinone and then to benzoquinone in the aqueous extracts was analyzed quantitatively by HPLC. The changes in the contents of benzoquinone in the extracts of leaves and stems with tissue aging and with increase in time after preparation of the extracts paralleled the changes in antibacterial activity as determined by the quantitative bioassay.
机译：梨Pyrus spp的多汁幼芽组织的水提物。对细菌欧文氏菌（Erwinia amylovora bv）具有较强的抗菌活性。 4.通过新开发的生物测定方法定量研究了这种活性。发现该活性随组织的年龄而变化。枝梢上最年轻的叶和茎的提取物显示出最强的活性，并且活性随着叶和茎的年龄而降低。制备提取物后，活性也随时间的增加而变化，在制备后的第一小时内迅速增加，在约4 h达到最大值，然后缓慢降低。通过真空中的蒸汽蒸馏并通过木炭粉末柱色谱法从提取物中分离出对抗菌活性必不可少的物质。通过NMR光谱，质谱和HPLC分析鉴定为苯醌（2，5-环己二烯-1，4-二酮）。通过HPLC定量分析了水提取物中熊果苷到对苯二酚再到苯醌的酚代谢。随着组织老化和提取物制备后时间的延长，叶和茎中提取物中苯醌的含量变化与通过定量生物测定法确定的抗菌活性变化平行。

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