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首页> 外文期刊>Phytochemistry >Identification of anti-inflammatory constituents in Hypericum perforatum and Hypericum gentianoides extracts using RAW 264.7 mouse macrophages.
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Identification of anti-inflammatory constituents in Hypericum perforatum and Hypericum gentianoides extracts using RAW 264.7 mouse macrophages.

机译：使用RAW 264.7小鼠巨噬细胞鉴定贯叶连翘和龙胆提取物中的抗炎成分。

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Hypericum perforatum (St. John's wort) is an herb widely used as supplement for mild to moderate depression. Our prior studies established synergistic anti-inflammatory activity associated with 4 bioactive compounds in a fraction of a H. perforatum ethanol extract. Whether these 4 compounds also contributed to the ethanol extract activity was addressed in the research reported here. Despite the popularity of H. perforatum, other Hypericum species with different phytochemical profiles could have their anti-inflammatory potentials attributed to these or other compounds. In the current study, ethanol extracts of different Hypericum species were compared for their inhibitory effect on LPS-induced prostaglandin E2 (PGE2) and nitric oxide (NO) production in RAW 264.7 mouse macrophages. Among these extracts, those made from H. perforatum and H. gentianoides demonstrated stronger overall efficacy. LC-MS analysis established the 4 compounds were present in the H. perforatum extract and pseudohypericin in all active fractions. The 4 compounds accounted for a significant part of the extract's inhibitory activity on PGE2, NO, tumor necrosis factor- alpha (TNF- alpha ), and interleukin-1 beta (IL-1 beta ) in RAW 264.7 as well as peritoneal macrophages. Pseudohypericin was the most important contributor of the anti-inflammatory potential among the 4 compounds. The lipophilic fractions of H. gentianoides extract, which did not contain the previously identified active constituents, decreased PGE2 and NO potently. These fractions were rich in acylphloroglucinols, including uliginosin A that accounted for a proportion of the anti-inflammatory activity observed with the active fractions. Overall, the current study established that a different group of major anti-inflammatory constituents were present in H. gentianoides, while showing that the previously identified 4 compound combination was important for H. perforatum's anti-inflammatory potential.
机译：Hypericum perforatum （贯叶连翘）是一种广泛用作轻度至中度抑郁症的补充剂的草药。我们先前的研究建立了与4种生物活性化合物相关的协同抗炎活性，而这些化学活性仅占H的一小部分。贯叶连翘乙醇提取物。此处报道的研究探讨了这4种化合物是否也有助于乙醇提取物的活性。尽管 H很流行。 perforatum ，其他具有不同植物化学特征的er> Hypericum 物种，其抗炎潜力可能归因于这些或其他化合物。在当前的研究中，比较了不同的金丝桃属植物乙醇提取物对RAW 264.7小鼠巨噬细胞中LPS诱导的前列腺素E2（PGE2）和一氧化氮（NO）产生的抑制作用。在这些提取物中，由H制成。 perforatum 和 H。龙胆甙显示出更强的整体功效。 LC-MS分析确定4种化合物存在于H中。穿孔提取物和伪高丝菌素的所有有效成分。这四种化合物在RAW 264.7和腹膜巨噬细胞中对PGE2，NO，肿瘤坏死因子α（TNF-α）和白细胞介素1β（IL-1 beta）的抑制作用中占很大一部分。伪高丝菌素是这四种化合物中最重要的抗炎药。 H的亲脂级分。龙胆提取物不含先前确定的活性成分，可有效降低PGE2和NO含量。这些馏分富含酰基葡糖醛酸，其中包括芥子苷A，占活性馏分中观察到的抗炎活性的一部分。总体而言，当前研究确定H中存在不同种类的主要抗炎成分。龙胆甙，同时表明先前确定的4种化合物组合对 H很重要。贯叶连翘的抗炎潜力。

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