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首页> 外文期刊>Phytochemistry >Increased accumulation of anthocyanins in Fragaria chiloensis fruits by transient suppression of FcMYB1 gene.
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Increased accumulation of anthocyanins in Fragaria chiloensis fruits by transient suppression of FcMYB1 gene.

机译：通过瞬时抑制FcMYB1基因，增加草莓草莓果实中花色苷的积累。

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摘要

Anthocyanins and proanthocyanidins (PAs), flavonoid-derived metabolites with different physiological roles, are produced by plants in a coordinated manner during fruit development by the action of transcription factors (TFs). These regulatory proteins have either an activating or repressing effect over structural genes from the biosynthetic pathway under their control. FaMYB1, a TF belonging to the R2R3-MYB family and isolated from commercial strawberry fruit (Fragaria x ananassa), was reported as a transcriptional repressor and its heterologous over-expression in tobacco flowers suppressed flavonoid-derived compound accumulation. FcMYB1, an ortholog of FaMYB1 isolated from the white Chilean strawberry (Fragaria chiloensis ssp. chiloensis f. chiloensis), showed higher transcript levels in white (F. chiloensis) than in red (F. x ananassa cv. Camarosa) fruits. In order to assess its contribution to the discolored phenotype in F. chiloensis, FcMYB1 was transiently down-regulated in planta using an RNAi-based approach. Quantitative real-time PCR on FcMYB1 down-regulated fruits resulted an up-regulation of anthocyanidin synthase (ANS) and a strong repression of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) transcript accumulation. In addition, these fruits showed increased concentrations of anthocyanins and undetectable levels of flavan 3-ols. Altogether, these results indicate a role for FcMYB1 in regulation of the branching-point of the anthocyanin/PA biosynthesis determining the discolored phenotype of the white Chilean strawberry fruit
机译：花青素和原花青素（PAs）是具有不同生理作用的类黄酮衍生代谢产物，是植物在果实发育过程中通过转录因子（TFs）的作用以协调的方式产生的。这些调节蛋白对来自其控制下的生物合成途径的结构基因具有激活或抑制作用。 FaMYB1，属于R2R3-MYB家族的TF，从商业草莓果实（Fragaria x ananassa）中分离，据报道是转录阻遏物，其在烟草花中的异源过表达抑制了类黄酮衍生的化合物积累。从白色智利草莓（Fragaria chiloensis ssp。chiloensis f。chiloensis）分离得到的FaMYB1直系同源物FcMYB1，在白色（F. chiloensis）中的转录水平高于红色（F. x ananassa cv。Camarosa）果实。为了评估其对Chiloensis变色表型的贡献，使用基于RNAi的方法在植物体内瞬时下调了FcMYB1。 FcMYB1下调的果实的实时定量PCR导致花色素苷合酶（ANS）上调，并强烈抑制花色素苷还原酶（ANR）和无色花色素还原酶（LAR）转录产物的积累。此外，这些果实显示出花青素浓度增加，而黄烷3-ol含量未检出。总而言之，这些结果表明FcMYB1在花青素/ PA生物合成分支点调节中的作用，决定了白色智利草莓果实的变色表型。

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