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首页> 外文期刊>Biomaterials >The differential effect of scaffold composition and architecture on chondrocyte response to mechanical stimulation.
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The differential effect of scaffold composition and architecture on chondrocyte response to mechanical stimulation.

机译：支架组成和结构对软骨细胞对机械刺激的反应的差异作用。

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This study aims to explore the differential effect of scaffold composition and architecture on chondrogenic response to dynamic strain stimulation using encapsulating PEG-based hydrogels and primary bovine chondrocytes. Proteins and proteoglycans were conjugated to functionalized poly(ethylene glycol) (PEG) and immobilized in PEG hydrogels to create bio-synthetic materials to be used as scaffolds. Four different compositions were tested, including: PEG-Proteoglycan (PP), PEG-Fibrinogen (PF), PEG-Albumin (PA), and PEG only. Primary articular chondrocytes were encapsulated in the hydrogel scaffolds and subjected to 15% dynamic compressive strain stimulation at 1-Hz frequency for 28 days. Stimulation of PP, PF, PA and PEG constructs resulted in a respective increase in the unconfined true compressive modulus by 32%, 45.4%, 33.6%, and 28.2%, compared to their static controls. The PF showed a significantly larger relative increase in the modulus in comparison to all other scaffolds tested. These results support the hypothesis that mechanical stimulation and material bioactivity have a significant effect on the reported chondrocyte response. Similar trends were observed with the swelling ratio of the constructs. These findings indicate that while stimulation causes metabolic changes in chondrocytes seeded in PEG hydrogels, the matrix bioactivity has a significant role in enhancing chondrocyte mechanotransduction in encapsulating scaffolds subjected to physical deformations.
机译：这项研究旨在探索支架组成和结构对软骨细胞对动态应变刺激的软骨形成反应的不同影响，使用封装的基于PEG的水凝胶和原代牛软骨细胞。将蛋白质和蛋白聚糖与功能化的聚乙二醇（PEG）偶联，并固定在PEG水凝胶中，以创建用作支架的生物合成材料。测试了四种不同的成分，包括：PEG-蛋白聚糖（PP），PEG-纤维蛋白原（PF），PEG-白蛋白（PA）和仅PEG。将原发性关节软骨细胞封装在水凝胶支架中，并以1 Hz的频率进行15％动态压缩应变刺激，持续28天。 PP，PF，PA和PEG构造物的刺激导致其无侧限真实压缩模量与其静态对照相比分别增加了32％，45.4％，33.6％和28.2％。与测试的所有其他支架相比，PF的模量相对增加幅度明显更大。这些结果支持以下假设：机械刺激和物质生物活性对所报道的软骨细胞反应具有显着影响。在构建体的溶胀率方面观察到相似的趋势。这些发现表明，虽然刺激引起接种在PEG水凝胶中的软骨细胞的代谢变化，但是基质生物活性在增强遭受物理变形的封装支架中的软骨细胞机械转导中具有重要作用。

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