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首页> 外文期刊>Photochemistry and Photobiology: An International Journal >Time-resolved singlet oxygen phosphorescence measurements from photosensitized experiments in single cells: Effects of oxygen diffusion and oxygen concentration
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Time-resolved singlet oxygen phosphorescence measurements from photosensitized experiments in single cells: Effects of oxygen diffusion and oxygen concentration

机译：单细胞光敏实验中的时间分辨单线态氧磷光度测量：氧扩散和氧浓度的影响

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摘要

Time-resolved singlet oxygen, O-2(a(1)Delta(g)), phosphorescence experiments have been performed in single cells upon pulsed laser irradiation of a photosensitizer incorporated into the cell. Data recorded as a function of the partial pressure of ambient oxygen to which the cell is exposed reflect apparent values for the intracellular oxygen diffusion coefficient and intracellular oxygen concentration that are smaller than those found in neat H2O. This conclusion is supported by O-2(a(1)Delta(g)) phosphorescence data and sensitizer triplet state absorption data recorded in control experiments on sucrose solutions with different viscosities. We recently demonstrated that the intracellular lifetime of O-2(a(1)Delta(g)) is comparatively long (similar to 3 mu s) and does not differ significantly from that in neat H2O (similar to 3.5 mu s). Despite this long lifetime, however, our estimate of an apparent intracellular oxygen diffusion coefficient in the range similar to 2-4 x 10(-6) cm(2) s(-1) means that the spatial domain of intracellular O-2(a(1)Delta(g)) activity will likely have a spherical radius of similar to 100 nm. This latter point helps reconcile seeming inconsistencies between our direct O-2(a(1)Delta(g)) lifetime data and results obtained from independent photobleaching experiments that show a limited translational diffusion distance for O-2(a(1)Delta(g)) within a cell.
机译：时间分辨的单线态氧O-2（a（1）Delta（g））的磷光实验是在掺入电池的光敏剂的脉冲激光辐照下在单个电池中进行的。记录为细胞暴露于其中的环境氧分压的函数的数据，反映出细胞内氧扩散系数和细胞内氧浓度的表观值小于纯净水中的那些。在不同粘度的蔗糖溶液的对照实验中记录的O-2（a（1）Delta（g））磷光数据和敏化剂三重态吸收数据支持了该结论。我们最近证明，O-2（a（1）Delta（g））的细胞内寿命相对较长（大约3 s），并且与纯净水（大约3.5 s）没有明显差异。尽管寿命长，但我们估计表观细胞内氧扩散系数在类似于2-4 x 10（-6）cm（2）s（-1）的范围内，这意味着细胞内O-2（ a（1）Delta（g））活性的球形半径可能类似于100 nm。后一点有助于调和我们的直接O-2（a（1）Delta（g））寿命数据与独立光漂白实验获得的结果之间的似乎不一致之处，该结果表明O-2（a（1）Delta（ g））。

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