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首页> 外文期刊>Photochemistry and Photobiology: An International Journal >CELL CYCLE EFFECTS AND CONCOMITANT P53 EXPRESSION IN HAIRLESS MURINE SKIN AFTER LONGWAVE UVA (365 NM) IRRADIATION - A COMPARISON WITH UVB IRRADIATION
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CELL CYCLE EFFECTS AND CONCOMITANT P53 EXPRESSION IN HAIRLESS MURINE SKIN AFTER LONGWAVE UVA (365 NM) IRRADIATION - A COMPARISON WITH UVB IRRADIATION

机译：紫外线（365 NM）照射后无毛鼠皮肤细胞周期效应和P53的同时表达-紫外线照射的比较。

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Ultraviolet A (UVA, 315-400 nm) radiation is known to be a complete carcinogen, but in contrast to UVB (280-315 nm) radiation, much of the cell damage is oxygen dependent (mediated through reactive oxygen species), and the dominant premutational DNA lesion(s) remains to be identified, To investigate further the basic differences in UVA and UVB carcinogenesis, we compared in vivo cellular responses, viz. cell cycle progression and transient p53 expression in the epidermis, after UVA1 (340-400 nm) exposure with those after broadband UVB exposure of hairless mice, Using flow cytometry we found a temporary suppression of bromodeoxyuridine (BrdU) uptake in S-phase cells both after UVB and UVA1 irradiation, which only in the case of UVB is followed by an increase to well over control levels. With equally erythemogenic doses (1-2 MED), the modulation of BrdU uptake was more profound after UVB than after UVA1 irradiation, Also, a marked transient increase in the percentage of S-phase cells occurred both after UVB and after UVA1 irradiation, but this increase evolved more rapidly after UVA1 irradiation, Further, p53 expression increased both after UVB and UVA1 irradiations, with peak expression already occurring from 12 to 24 h after UVA1 exposure and around 24 h after UVB exposure, Overall, UVA1 radiation appears to have less of an impact on the cell cycle than UVB radiation, as measured by the magnitude and duration of changes in DNA synthesis and cells in S phase, These differences are likely to reflect basic differences between UVB and UVA1 in genotoxicity and carcinogenic action. [References: 37]
机译：已知紫外线A（UVA，315-400 nm）辐射是一种完全的致癌物，但与UVB（280-315 nm）辐射相反，许多细胞损伤是氧依赖性的（通过活性氧物质介导），并且尚待确定主要的突变前DNA损伤。为进一步研究UVA和UVB致癌作用的基本差异，我们比较了体内细胞反应，即。 UVA1（340-400 nm）暴露后和无毛小鼠宽带UVB暴露后表皮细胞周期变化和瞬时p53表达在UVB和UVA1辐照后，仅在UVB情况下才增加到远远超过控制水平。在相同的造血剂量（1-2 MED）下，UVB后对BrdU摄取的调节比UVA1辐照后更深。此外，UVB后和UVA1辐照后，S期细胞百分比均出现明显的瞬时增加，但在UVA1照射后，这种增加更快地发展。此外，在UVB1和UVA1照射后，p53表达均增加，在UVA1暴露后12至24 h和UVB暴露后24 h左右已经出现了峰值表达，总的来说，UVA1辐射似乎较少通过DNA合成和S期细胞变化的幅度和持续时间来衡量，与UVB辐射相比对细胞周期的影响，这些差异很可能反映了UVB和UVA1在遗传毒性和致癌作用方面的基本差异。 [参考：37]

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