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Biosensors Based on Cross-Linking of Biotinylated Glucose Oxidase By Avidin

机译：生物传感器基于生物素化葡萄糖氧化酶交联的抗生物素蛋白

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摘要

Avidin, having four biotin binding units, cross-links and immobilizes glucoseoxidase (GOX) labeled with multiple biotins (B-GOX) when solutions of B-GOX and avidin are mixed on an electrode. The H2O2 flux generated in the B-GOX catalyzed oxidation of glucose by dissolved O2 is measured as an electroreduction current at a horseradish peroxidase (HRP) redox conducting hydrogel electrode poised at +100 mV (Ag/AgCl). The sensitivity of the resulting glucose sensor is 0.14A/sq cm/M with a linear range up to 2mM glucose. (jg).

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作者
Vreeke, M. S.; Rocca, P.;
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年度 1995
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总页数 20
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Crosslinking(Chemistry); Biological detection; Glucose; Vitamin B complex; Biochemistry; Enzymes; Oxidation; Electrodes; Catalysis; Electric current; Gels; Peroxidases;

机译：交联（化学）;生物学检测;葡萄糖;维生素B复合物;生物化学;酶;氧化;电极;催化;电流;凝胶;过氧化物酶;

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3. Glucose biosensor based on the immobilization of glucose oxidase on electrochemically synthesized polypyrrole-poly(vinyl sulphonate) composite film by cross-linking with glutaraldehyde [J] . ?zlem ?olak, Ahmet Ya?ar, Servet ?ete, Artificial cells, blood substitutes, and immobilization biotechnology. . 2012,第5期

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4. A self-powered glucose biosensor based on pyrolloquinoline quinone glucose dehydrogenase and bilirubin oxidase operating under physiological conditions [C] . Tanmay Kulkarni, Gymama Slaughter Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2017

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5. The development and application of glucose electrodes based on "wired" glucose oxidase. [D] . Chen, Ting. 2001

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6. Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite [O] . Sabriye Yusan, Mokhlesur M. Rahman, Nasir Mohamad, 2018

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7. Enzyme Multilayer-Modified Biosensors. Use of Streptavidin and Deglycosylated Avidin for Constructing Glucose Oxidase and Lactate Oxidase Multilayers. [O] . Jun-ichi ANZAI, Yuka KOBAYASHI, Hiroki TAKESHITA 1997

机译：酶多层改性的生物传感器。用链霉抗生物素蛋白和脱糖基化的抗生物素蛋白用于构建葡萄糖氧化酶和乳酸氧化酶多层。

Biosensors Based on Cross-Linking of Biotinylated Glucose Oxidase By Avidin

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