• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国政府科技报告 >Secretome Biomarkers for the Identification and Differentiation of Enterohemorrhagic and Enteropathogenic Escherichia coli Strains.
【24h】

Secretome Biomarkers for the Identification and Differentiation of Enterohemorrhagic and Enteropathogenic Escherichia coli Strains.

机译：用于鉴定和分化肠出血性和肠致病性大肠杆菌菌株的secretome生物标志物。

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The secreted proteins of the enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) are the most common cause of hemorrhagic colitis, which is a bloody diarrhea with EHEC infection that can often lead to lifethreatening hemolytic-uremic syndrome. We are employing a metaproteomic approach as an effective and complementary technique to the current genomic- based approaches. This metaproteomic approach will evaluate the secreted proteins associated with pathogenicity and utilize their signatures as differentiation biomarkers between EHEC and EPEC strains. Analysis of extract from EHEC O104:H4 resulted in the identification of a multidrug efflux protein that belongs to the family of fusion proteins, which are responsible for cell transportation. The experimental peptides identified lie in the region of the HlyD hemolysin secretion protein-D, which is responsible for transporting the hemolysin A toxin. Moreover, the taxonomic classification of EHEC O104:H4 showed the closest match with E. coli E55989, which is in agreement with genomic-sequencing studies that were done extensively on the aforementioned strain. Comparative proteomic calculations showed separation between EHEC O157:H7 and O104:H4 in replicate samples using cluster analysis. There were no reported studies that addressed the characterization of secreted proteins in various enhanced-growth media and utilized them as biomarkers for strain differentiation.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号