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首页> 美国政府科技报告 >Immunodetection of T-2 Metabolites in Rat Urines after Dermal, Oral, or Intramuscular Exposure to T-2 Toxin
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Immunodetection of T-2 Metabolites in Rat Urines after Dermal, Oral, or Intramuscular Exposure to T-2 Toxin

机译：皮肤，口服或肌肉注射T-2毒素后大鼠尿液中T-2代谢物的免疫检测

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T-2 toxin metabolites were detected in rat urines up to 2 weeks after the animals' exposure to sublethal dose of T-2 toxin. Urines were assayed for HT-2 or T-2 tetraol by radioimmunoassay (RIA) with polyclonal antibody. Sensitivity of the RIA was 5 nanogram/ml for HT=2 and 50 nanogram/ml for T-2 tetraol. Some urines were assayed in parallel with an enzyme-linked immunosorbent assay (ELISA) developed for T-2 with a monoclonal antibody that cross reacts with HT-2. The sensitivity of the ELISA for detection of HT-2 in rat urines was 250 nanogram/ml. Urines were diluted 1:2 or 1:5 to eliminate high backgrounds, which appeared as false positives in the T-2 tetraol RIA and ELISA assays. T-2 or its metabolites could be detected up to 96 hrs after an im injection of T-2 toxin. In rats exposed orally or dermally, metabolites were detected up to 10 or 16 days, respectively. 'T-2 equivalents' detected by the ELISA correlated with the HT-2 or HT-2-like compounds detected by RIA. Keywords: Tusarium, Phytotoxins, Immunoassay. (kt)

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