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首页> 美国政府科技报告 >Application of Sister Chromatid Exchange in Marine Polychaetes to Black Rock Harbor Sediment. Laboratory Documentation Phase
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Application of Sister Chromatid Exchange in Marine Polychaetes to Black Rock Harbor Sediment. Laboratory Documentation Phase

机译：姊妹染色单体交换在海洋多毛类植物中对黑岩港沉积物的应用。实验室文件阶段

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This report presents an evaluation of the applicability of the cytogenetic technique of sister chromatid exchange (SCE) to measure genotoxic effects of highly contaminated dredged material, and the degree of variability and reproducibility inherent in the procedure. This project is part of the US Environmental Protection Agency/Corps of Engineers Field Verification Program. The SCE technique was applied to Nephtys incisa, an infaunal polychaete dominant in the benthic community at the Central Long Island Sound disposal site. The SCE response was measured in N. incisa exposed to suspended particulate and bedded phases of Black Rock Harbor (BRH) sediment in the laboratory. Neanthes arenaceodentata, a surrogate spcies, was tested in parallel to N. incisa. With the exception of one treatment in one experiment, the worm chromosomes were uniformly nonresponsive to BRH sediment. Replicate treatments within an experiment did not differ significantly for N. arenaceodentata. Differences between experiments and between species within a experiment were found. The reason for the differences are known. Differences in ability to metabolize polynuclear aromatic hydrocarbons, found in high concentrations in BRH sediments, is a likely but specalative reason. Clearly, additional research is needed before SCE could be used for routine testing.

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