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ELISA (Enzyme-linked Immunosorbent Assay) to Detect Humoral Antibodies Specific for Clostridium botulinum Type A Neurotoxin

机译：ELIsa（酶联免疫吸附试验）检测特异性肉毒杆菌a型神经毒素的体液抗体

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摘要

The enzyme-linked immunosorbent assay (ELISA) was modified for the rapid detection of human humoral antibodies (IgG) that are specific for Clostridium botulinum type a toxoid. The ELISA was useful for the rapid evaluation of immunization results and for distinguishing (95%) immune from nonimmune individuals. ELISA values, when compared with standard in vivo toxin neutralization test values, paralleled the anamnestic response to booster immunization in 25 to 25 individuals.

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Lewis, G. E.; Kulinski, S. S.; Metzger, J. F.; Higbee, G. A.;
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年度 1985
页码 p.1-19
总页数 19
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Clostridium botulinum; Botulism; Diseases; Food poisoning; Immunization; Enzymes; Immunoassay; Antibodies; Humoral immunity; Detection; Quick reaction; Toxins and antitoxins; Humans; Cns depressants; Test and evaluation;

机译：肉毒杆菌;肉毒杆菌;疾病;食物中毒;免疫;酶;免疫测定;抗体;体液免疫;检测;快速反应;毒素和抗毒素;人类; Cns抑制剂;测试和评估;

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1. Enzyme-linked immunosorbent assay and enzyme-linked coagulation assay for detection of Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes with dual-label antibodies. [J] . G J Doellgast, G A Beard, J D Bottoms, Journal of Clinical Microbiology . 1994,第1期

机译：酶联免疫吸附测定和酶联凝血测定，用于检测肉毒梭菌神经毒素A，B和E以及具有双标记抗体的溶液相复合物。
2. Detection of Type A, B, E, and F Clostridium botulinum Neurotoxins in Foods by Using an Amplified Enzyme-Linked Immunosorbent Assay with Digoxigenin-Labeled Antibodies [J] . Shashi K. Sharma, Joseph. L. Ferreira, Brian S. Eblen, Applied and Environmental Microbiology . 2006,第2期

机译：通过使用洋地黄毒苷标记的抗体的酶联免疫吸附试验，检测食品中的A，B，E和F型肉毒梭菌神经毒素
3. Enzyme-linked immunosorbent assay-enzyme-linked coagulation assay for detection of antibodies to Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes. [J] . G J Doellgast, M X Triscott, G A Beard, Journal of Clinical Microbiology . 1994,第3期

机译：酶联免疫吸附测定法-酶联凝血测定法，用于检测肉毒梭菌神经毒素A，B和E抗体以及溶液相复合物。
4. PORCINE CIRCOVIRUS TYPE 2 : ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETECTION OF ANTIGEN AND ANTIBODIES IN FECES [C] . P Lopez, E Deshaies, N Brajon, Congress of the International Pig Veterinary Society . 2002

机译：猪圆环病毒2型：用于检测粪便中的抗原和抗体的酶联免疫吸附试验（ELISA）
5. Development of a monoclonal antibody-based antigen detection enzyme-linked immunosorbent assay (ELISA) for the diagnosis of human toxoplasmosis. [D] . Grushka, Daniel. 2001

机译：基于单克隆抗体的抗原检测酶联免疫吸附测定（ELISA）的开发，用于诊断人类弓形虫病。
6. Enzyme-linked immunosorbent assay and enzyme-linked coagulation assay for detection of Clostridium botulinum neurotoxins A B and E and solution-phase complexes with dual-label antibodies. [O] . G J Doellgast, G A Beard, J D Bottoms, 1994

机译：酶联免疫吸附测定和酶联凝血测定用于检测肉毒梭菌神经毒素AB和E以及具有双标记抗体的溶液相复合物。
7. Detection of Type A, B, E, and F Clostridium botulinum Neurotoxins in Foods by Using an Amplified Enzyme-Linked Immunosorbent Assay with Digoxigenin-Labeled Antibodies [O] . Sharma, Shashi K., Ferreira, Joseph. L., Eblen, Brian S., 2006

机译：使用洋地黄毒苷标记抗体的扩增酶联免疫吸附法检测食品中的A，B，E和F型肉毒梭菌神经毒素

ELISA (Enzyme-linked Immunosorbent Assay) to Detect Humoral Antibodies Specific for Clostridium botulinum Type A Neurotoxin

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