首页> 美国政府科技报告 >Monoclonal Antibodies that Recognize Proteins Unique to Somatic Embryos of 'Daucus carota'
【24h】

Monoclonal Antibodies that Recognize Proteins Unique to Somatic Embryos of 'Daucus carota'

机译:识别'Daucus carota'体细胞胚特有的蛋白质的单克隆抗体

获取原文

摘要

The authors have developed a panel of monoclonal antibodies (Mabs) to several antigens of Daucus carota somatic embryos. The authors prepared over 2400 hybridoma cultures from splenocytes of Balb/c mice immunized with lyophilized somatic embryo cells. The MAbs were screened on embryo, callus cell, and chloroplast extracts using an automated sampling system and computer-assisted analyses. Most of the MAbs reacted with antigens common to embryo and callus cells, nut MAbs from al least 5 hybridoma lines recognized single antigens unique to the somatic embryos. Other lines produced antibodies to chloroplast antigens, including the light-harvesting chlorophyll protein (LHCP).

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号