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Dry Aerosol Deposition Device (DADD): An Instrument for Depositing Microbial Aerosols onto Surfaces

机译:干气溶胶沉积装置(DaDD):一种将微生物气溶胶沉积到表面上的仪器

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The advent -- 60 years after the concept was first proposed -- of practical self-decontaminating materials coincides with international efforts to prepare for global viral epidemics to highlight a need for a method to rapidly and reproducibly contaminate surfaces with pathogenic bioaerosol particles and representative surrogates. Slow rotation of a single collection stage in a cascade impactor downstream of a Collison nebulizer is demonstrated to deposit by impaction a rotationally symmetric distribution of particles containing a single spore of Bacillus globigii or vegetative Staphylococcus aureus bacterium. The coefficient of variation observed for deposition on glass plates was approx. 14%, significantly less than the acceptance criterion of 20%, and deposition time is approx. 20 min. Particle size distribution can be manipulated -- by changing the nozzle in the Collison, by changing the concentration of inert organics added to the atomization mixture, or both -- suggesting a possibility of selectively depositing larger particles containing multiple organisms.

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