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Detection and Identification of Bacteria Using Automated Biological Sample Processing With a Relational Database Management System

机译:利用关系数据库管理系统自动生物样品处理检测和鉴定细菌

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The biological sample processing system (BSPS) combines sample pretreatment, protein concentration, and separation modules and was interfaced to electrospray ionization mass spectrometry (ESI-MS). Multivariate analysis software capabilities were used for automated data processing. The bacterial identification by the BSPS-MS is based on Liquid chromatography-mass spectrometry analysis of various bacterial proteins followed by their correlation with in-house database constructed from several bacteria of interest using our in-house ProMAPDB software. The identification of bacteria using ProMAPDB was validated using standard proteins analyzed by the BSPS-MS under various conditions. The results show that BSPS analysis was reproducible and sensitive at 1000 cfu/mL.

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