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Development of a Quantitative Real-Time Polymerase Chain Reaction Assay Specific for Orientia Tsutsugamushi

机译：恙虫病东方体的特异性实时聚合酶链反应检测方法的建立

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Two specific and sensitive polymerase chain reaction (PCR) assays were developed to detect and quantitate Orientia tsutsugamushi, the agent of scrub typhus, using a portion of the 47-kD outer membrane protein antigen/ high temperature requirement A gene as the target. A selected 47-kD protein gene primer pair amplified a 118-basepair fragment from all 26 strains of O. tsutsugamushi evaluated, but it did not produce amplicons when 17 Rickettsia and 18 less-related bacterial nucleic acid extracts were tested. Similar agent specificity for the real-time PCR assay, which used the same primers and a 31- basepair fluorescent probe, was demonstrated. This sensitive and quantitative assay determination of the content of O. tsutsugamushi nucleic acid used a plasmid containing the entire 47-kD gene from the Kato strain as a standard. Enumeration of the copies of O. tsutsugamushi DNA extracted from infected tissues from mice and monkeys following experimental infection with Orientia showed 27 5,552 copies/ L of mouse blood, 14,448 86,012 copies/ L of mouse liver/spleen homogenate, and 3 21 copies/ L of monkey blood.

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Jiang, J. ; Chan, T. ; Temenak, J. J. ; Dasch, G. A. ; Ching, W.;
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年度 2004
页码 1-7
总页数 7
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Immunoassay; Rickettsia tsutsugamushi; Reprints; Scrub typhus; Deoxyribonucleic acids; Plasmids;

机译：免疫分析;恙虫病立克次体;重印;丛林斑疹伤寒;脱氧核糖核酸;质粒;

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1. DEVELOPMENT OF A QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION ASSAY SPECIFIC FOR ORIENTIA TSUTSUGAMUSHI. [J] . Richards AL The American Journal of Tropical Medicine and Hygiene . 2004,第4期

机译：东方菊的定量实时聚合酶链反应分析的开发。
2. Short report: detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction. [J] . Singhsilarak T, Leowattana W, Looareesuwan S, The American Journal of Tropical Medicine and Hygiene . 2005,第5期

机译：简短报告：通过定量实时聚合酶链反应检测临床样品中的Or虫东方虫。
3. Specificity of a quantitative real-time polymerase chain reaction assay for the detection of invasive pneumococcal disease: identifying streptococcus pneumoniae using quantitative polymerase chain reaction. [J] . Kee C, Fatovich DM, Palladino Chest: The Journal of Circulation, Respiration and Related Systems . 2010,第1期

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4. Development of a Fluorescent Quantitative Real-Time Polymerase Chain Reaction Assay for the Detection of HSL Expression In Vitro [C] . Liu Zuo-Hua, Yang Jin-Long, Yang Fei-Yun, 5th International Conference on Bioinformatics and Biomedical Engineering . 2011

机译：用于体外检测HSL表达的荧光定量实时聚合酶链反应测定方法的开发
5. Development and application of a real-time polymerase chain reaction assay for the myxozoan parasite Henneguya ictaluri. [D] . Griffin, Matthew John. 2008

机译：粘虫寄生虫Henneguya ictaluri的实时聚合酶链反应测定方法的开发和应用。
6. Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2 [O] . Yang Zhang, Chunyang Dai, Huiyan Wang, 2020

机译：高度敏感的一步嵌套定量实时聚合酶链反应测定的分析与验证进行特异性急性呼吸综合征冠状病毒2的特异性检测
7. Development of a time-effective and highly specific quantitative real-time polymerase chain reaction assay for the identification of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in artisanal raw cow’s milk cheese [O] . Milena Alicja Stachelska, Roberta Foligni 2018

机译：开发时间有效且高度特异性的定量实时聚合酶链反应测定，用于鉴定乳酸杆菌Delbrueckii子公司。保加利宫和链球菌热管在手工制造牛奶奶酪

Development of a Quantitative Real-Time Polymerase Chain Reaction Assay Specific for Orientia Tsutsugamushi

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