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Genetically Engineered, Live Attenuated Vaccines for Venezuelan Equine Encephalitis: Testing in Animal Models

机译：基因工程，委内瑞拉马脑炎减毒疫苗：动物模型测试

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The central objective of this research was to test molecularly defined, live attenuated Venezuelan equine encephalitis virus (VEEV) vaccine candidates that were produced through precise genetic manipulation of rationally selected viral nucleotide sequences. Molecular clones of vaccine candidates were constructed by inserting either three independently attenuating mutations or a PE2 cleavage-signal mutation with a second-site resuscitating mutation into full-length cDNA clones. Vaccine candidate viruses were recovered through DNA transcription and RNA transfection of cultured cells, and assessed in rodent and non-human primate models. Based on results from this assessment, one of the PE2 cleavage-signal mutants, V3526, was determined to be the best vaccine candidate for further evaluation for human use.

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Pratt, W. D. ; Davis, N. L. ; Johnston, R. E. ; Smith, J. F.;
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年度 2003
页码 1-10
总页数 10
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Genetics; Vaccines; Laboratory animals; Venezuelan equine encephalomyelitis virus; Reprints; Aerosols; Humans; Deoxyribonucleic acids; Mutations; Sequences; Attenuation; Clones; Primates; Exposure(Physiology); Rodents; Viruses; Ribonucleic acids; Nucleotides; Transfection;

机译：遗传学;疫苗;实验室动物;委内瑞拉马脑脊髓炎病毒;重印;气雾剂;人类;脱氧核糖核酸;突变;序列;衰减;克隆;灵长类动物;暴露（生理学）;啮齿动物;病毒;核糖核酸;核苷酸;转染;

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1. Genetically engineered, live attenuated vaccines for Venezuelan equine encephalitis: testing in animal models [J] . Pratt WD, Davis NL, Johnston RE, Vaccine . 2003,第25a26期

机译：委内瑞拉马脑炎的转基因减毒活疫苗：在动物模型中的测试
2. Genetically engineered, live, attenuated vaccines protect nonhuman primates against aerosol challenge with a virulent IE strain of Venezuelan equine encephalitis virus [J] . Reed DS, Lind CM, Lackemeyer MG, Vaccine . 2005,第24期

机译：转基因的减毒活疫苗通过委内瑞拉马脑炎病毒的强毒IE株保护非人类灵长类动物免受气溶胶攻击
3. Limited potential for mosquito transmission of genetically engineered, live-attenuated Venezuelan equine encephalitis virus vaccine candidates. [J] . Turell MJ, Ludwig GV, Kondig J, The American Journal of Tropical Medicine and Hygiene . 1999,第6期

机译：转基因的，减毒的委内瑞拉马脑炎病毒候选疫苗的蚊子传播潜力有限。
4. Comparative Epidemiologies of Eastern Equine Encephalitis Virus, Western Equine Encephalitis Virus, Venezuelan Equine Encephalitis Virus, and West Nile Virus [C] . Charles H. Calisher Annual Meeting of the American Society for Veterinary Clinical Pathology . 2004

机译：东部大雄脑炎病毒，西马脑炎病毒，委内瑞拉脑脑炎病毒和西尼罗河病毒的比较流行病学
5. Development of improved dengue 2 antigen configurations for a Venezuelan equine encephalitis virus replicon particle-launched dengue vaccine [D] . Webb, Drue Laine 2008

机译：委内瑞拉马脑炎病毒复制子颗粒登革热疫苗的改进登革2抗原构型的开发
6. A DNA Vaccine for Venezuelan Equine Encephalitis Virus Delivered by Intramuscular Electroporation Elicits High Levels of Neutralizing Antibodies in Multiple Animal Models and Provides Protective Immunity to Mice and Nonhuman Primates [O] . Lesley C. Dupuy, Michelle J. Richards, Barry Ellefsen, 2011

机译：肌内电穿孔递送委内瑞拉马脑炎病毒的DNA疫苗可在多种动物模型中激发高水平的中和抗体并为小鼠和非人类灵长类动物提供保护性免疫力。
7. LIMITED POTENTIAL FOR MOSQUITO TRANSMISSION OF GENETICALLY ENGINEERED, LIVE-ATTENUATED VENEZUELAN EQUINE ENCEPHALITIS VIRUS VACCINE CANDIDATES [O] . Michael J. Turell, George V. Ludwig, John Kondig, 2014

机译：对遗传工程，生物减毒的VENEZUELaN EQUINE ENCEpHaLITIs病毒疫苗候选者进行蚊子传播的有限潜力

Genetically Engineered, Live Attenuated Vaccines for Venezuelan Equine Encephalitis: Testing in Animal Models

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