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In Vivo DNA Binding Properties of Wild-type and Mutant p53 Proteins in Mammary Cell Lines During the Course of Cell Cycle

机译：细胞周期过程中乳腺细胞系中野生型和突变型p53蛋白的体内DNa结合特性

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Using centrifugal elutriation we have separated exponentially growing ML-1 cells into specific cell cycle populations and have treated these discrete fractions with Camptothecin for 6 hours. These experiments allowed us to determine that Camptothecin-induced apoptosis and activation of p53 Via stabilization and p53 phosphorylation occur in all stages of the cell cycle. We have also determined that p53 mediated activation of the mdm2 gene occurs from a constitutively nucleosome free region. Additionally we have observed that differential activation of p53 induced genes does not occur at the level of chromatin reorganization. Finally we have observed that mutant p53 and Spl can be coordinately purified from an Spl DNA affinity column.

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作者
Bargonetti, J. ; Fleissner, E.;
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年度 1998
页码 1-49
总页数 49
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
In vivo analysis; Cells(Biology); Breast cancer; Activation; Regions; Mammary glands;

机译：体内分析;细胞（生物学）;乳腺癌;激活;区域;乳腺;

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1. Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus. [J] . P K Orberg, P A Schaffer Journal of Virology . 1987,第4期

机译：单纯疱疹病毒1型主要DNA结合蛋白，ICP8，转化细胞系中的表达：缺失突变体的互补和野生型病毒的抑制作用。
2. Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus. [J] . P K Orberg, P A Schaffer Journal of Virology . 1987,第4期

机译：单纯疱疹病毒1型主要DNA结合蛋白，ICP8，转化细胞系中的表达：缺失突变体的互补和野生型病毒的抑制作用。
3. Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus. [J] . P K Orberg, P A Schaffer Journal of Virology . 1987,第4期

机译：单纯疱疹病毒1型主要DNA结合蛋白，ICP8，转化细胞系中的表达：缺失突变体的互补和野生型病毒的抑制作用。
4. PENTOXIFYLLINE INHIBITS THE IRRADIATION INDUCED G_2/M BLOCK AND ALTERS DNA SYNTHESIS IN P53 MUTANT AND REPAIR DEFICIENT CELLS [C] . NATO advanced research workshop on fundamentals for the assessment of risks from environmental radiation . 1999

机译：Pentoxifylline抑制辐照诱导的G_2 / m块，并改变P53突变体和修复缺陷细胞中的DNA合成
5. Determining the role of p53 mutation in human breast cancer progression using recombinant mutant/wild-type p53 heterozygous human mammary epithelial cell culture models. [D] . Junk, Damian Jerome. 2008

机译：使用重组突变体/野生型p53杂合性人类乳腺上皮细胞培养模型确定p53突变在人类乳腺癌进展中的作用。
6. Expression of herpes simplex virus type 1 major DNA-binding protein ICP8 in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus. [O] . P K Orberg, P A Schaffer 1987

机译：单纯疱疹病毒1型主要DNA结合蛋白ICP8在转化细胞系中的表达：缺失突变体的互补和对野生型病毒的抑制。
7. Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus [O] . P K Orberg, P A Schaffer 1987

机译：单纯疱疹病毒的表达1主要DNA结合蛋白，ICP8，转化细胞系：缺失突变体的互补和野生型病毒的抑制作用

In Vivo DNA Binding Properties of Wild-type and Mutant p53 Proteins in Mammary Cell Lines During the Course of Cell Cycle

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