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Subunits of Highly Fluorescent Protein R-phycoerythrin as Probes for Cell Imaging and Single Molecule Detection Doctoral thesis

机译：高荧光蛋白R-藻红蛋白亚基作为细胞成像和单分子检测的探针博士论文

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The purposes of our research were: 1. To characterize subunits of highly fluorescent protein R-Phycoerythrin (R-PE) and check their suitability for single-molecule detection (SMD) and cell imaging, 2. To extend the use of R-PE subunits through design of similar proteins that will be used as probes for microscopy and spectral imaging in a single cell, and 3. To demonstrate a high-throughput spectral imaging method that will rival spectral flow cytometry in the analysis of individual cells.

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作者
Isailovic, D.;
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年度 2005
页码 p.1-146
总页数 146
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Proteins; Imaging; Molecules; Detection; Cells; Proteins; Microscopy; Liquid chromatography; Characterization; Theses; Fluorescence; Mass spectrometry;

机译：蛋白质;成像;分子;检测;细胞;蛋白质;显微镜;液相色谱;表征;论文;荧光;质谱;

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1. Highly selective, naked-eye and fluorescent 'off-on' probe for detection of histidine/histidine-rich proteins and its application in living cell imagingf [J] . Shenyi Zhang, Chunmei Yang, Weiping Zhu, Organic & biomolecular chemistry . 2012,第8期

机译：用于检测组氨酸/富含组氨酸的蛋白质的高选择性，裸眼和荧光“关闭”探针及其在活细胞成像中的应用f
2. Highly fluorescent nitrogen-doped carbon dots derived from Phyllanthus acidus utilized as a fluorescent probe for label-free selective detection of Fe3+ ions, live cell imaging and fluorescent ink [J] . Raji Atchudan, Thomas Nesakumar Jebakumar Immanuel Edison, Kanikkai Raja Aseer, Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2018,第期

机译：高度荧光氮掺杂的碳点源自 Phyllanthus酸用于无标记选择性检测Fe 3 + 离子，活细胞成像和荧光墨水
3. Activation of Rac and Cdc42 Video Imaged by Fluorescent Resonance Energy Transfer-Based Single-Molecule Probes in the Membrane of Living Cells [J] . Reina E. Itoh, Kazuo Kurokawa, Yusuke Ohba, Molecular and Cellular Biology . 2002,第18期

机译：活化细胞膜中基于荧光共振能量转移的单分子探针对Rac和Cdc42视频的激活
4. Fluorescent pH probes, Fluorescent Proteins, and Intrinsic Cellular Fluorochromes are Tools to Study Cytosolic pH (pH~(cyt)) in Mammalian Cells [C] . Gloria M. Martinez, Lauren S. Gollahon, Keri Shafer, Society of Photo-Optical Instrumentation Engineers Conference on Biomarkers and Biological Spectral Imaging . 2001

机译：荧光pH探针，荧光蛋白和固有细胞荧光致荧光剂是在哺乳动物细胞中研究细胞溶胶pH（pH〜（cyt））的工具
5. Subunits of highly fluorescent protein R-phycoerythrin as probes for cell imaging and single-molecule detection. [D] . Isailovic, Dragan. 2005

机译：高荧光蛋白R-藻红蛋白的亚基，可作为细胞成像和单分子检测的探针。
6. Activation of Rac and Cdc42 Video Imaged by Fluorescent Resonance Energy Transfer-Based Single-Molecule Probes in the Membrane of Living Cells [O] . Reina E. Itoh, Kazuo Kurokawa, Yusuke Ohba, 2002

机译：基于荧光共振能量转移的单分子探针在活细胞膜中成像的Rac和Cdc42视频的激活。
7. Subunits of highly fluorescent protein R-phycoerythrin as probes for cell imaging and single-molecule detection [O] . Isailovic, Dragan 2005

机译：高荧光蛋白R-藻红蛋白的亚基作为细胞成像和单分子检测的探针

Subunits of Highly Fluorescent Protein R-phycoerythrin as Probes for Cell Imaging and Single Molecule Detection Doctoral thesis

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