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LDRD final report on microencapsulated immunoreagents for development of one-step ELISA

机译：LDRD关于微囊化免疫试剂的最终报告，用于开发一步法ELIsa

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Microencapsulation of biological macromolecules was investigated as a method for incorporating the necessary immunoreagents into an improved enzyme-linked immunosorbant assay (ELISA) package that would self-develop. This self-contained ELISA package would eliminate the need for a trained technician to perform multiple additions of immunoreagent to the assay. Microencapsulation by insolution drying was selected from the many available microencapsulation methods, and two satisfactory procedures for microencapsulation of proteins were established. The stability and potential for rapid release of protein from these microencapsulates was then evaluated. The results suggest that the chosen method for protein entrapment produces microcapsules with a considerable amount of protein in the walls making these particular microcapsules unsuitable for their intended use.

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