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Transformation-associated recombination between diverged and homologous DNA repeats is induced by strand breaks

机译：通过链断裂诱导分化和同源DNa重复之间的转化相关重组

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Rearrangement and deletion within plasmid DNA is commonly observed during transformation. We have examined the mechanisms of transformation-associated recombination in the yeast Saccharomyces cerevisiae using a plasmid system which allowed the effects of physical state and/or extent of homology on recombination to be studied. The plasmid contains homologous or diverged (19%) DNA repeats separated by a genetically detectable color marker. Recombination during transformation for covalently closed circular plasmids was over 100-fold more frequent than during mitotic-growth. The frequency of recombination is partly dependent on the method of transformation In that procedures involving lithium acetate or spheroplasting yield higher frequencies than electroporation. When present in the repeats, unique single-strand breaks that are ligatable, as well as double-strand breaks, lead to high levels of recombination between diverged and identical repeats. The transformation-associated recombination between repeat DNA's is under the influence of the RAD52, RAD1 and the RNC1 genes.

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Larionov, V. ; Kouprina, N. ; Eldarov, M. ; Perkins, E. ; Porter, G.;
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年度 1994
页码 1-28
总页数 28
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
DNA; Recombination; Saccharomyces cerevisiae; Genes; Plasmids; Strand Breaks;

机译：DNa;重组; saccharomyces cerevisiae;基因;质粒; strand Breaks;

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专利

1. Induction of recombination between homologous and diverged DNAs by double-strand gaps and breaks and role of mismatch repair. [J] . S D Priebe, J Westmoreland, T Nilsson-Tillgren, Molecular and Cellular Biology . 1994,第7期

机译：通过双链缺口和断裂诱导同源DNA和不同DNA之间的重组以及错配修复的作用。
2. Single-Strand Annealing, Conservative Homologous Recombination, Nonhomologous DNA End Joining, and the Cell Cycle-Dependent Repair of DNA Double-Strand Breaks Induced by Sparsely or Densely Ionizing Radiation [J] . Frankenberg-Schwager M, Gebauer A, Koppe C, Radiation Research: Official Organ of the Radiation Research Society . 2009,第3期

机译：单链退火，保守同源重组，非同源DNA末端连接以及稀疏或密集电离辐射诱导的DNA双链断裂的细胞周期依赖性修复
3. The Role of Nonhomologous DNA End Joining, Conservative Homologous Recombination, and Single-Strand Annealing in the Cell Cycle-Dependent Repair of DNA Double-Strand Breaks Induced by H2O2 in Mammalian Cells [J] . Frankenberg-Schwager M, Becker M, Garg I, Radiation Research: Official Organ of the Radiation Research Society . 2008,第6期

机译：非同源DNA末端连接，保守同源重组和单链退火在哺乳动物细胞中H2O2诱导的DNA双链断裂的细胞周期依赖性修复中的作用。
4. Repairing DNA double-strand breaks by the prokaryotic non-homologous end-joining pathway [C] . Nigel C. Brissett, Aidan J. Doherty Biochemical Society Symposium . 2009

机译：通过原核非同源终端连接途径修复DNA双链断裂
5. Regulation of the D-loop Intermediates in DNA Double-strand Break Repair by Homologous Recombination [D] . Shah, Shanaya Shital. 2020

机译：通过同源重组调节DNA双链断裂修复中的D环中间体
6. Induction of recombination between homologous and diverged DNAs by double-strand gaps and breaks and role of mismatch repair. [O] . S D Priebe, J Westmoreland, T Nilsson-Tillgren, 1994

机译：通过双链缺口和断裂诱导同源和不同DNA之间的重组以及错配修复的作用。
7. Transformation-associated recombination between diverged and homologous DNA repeats is induced by strand breaks [O] . V. Larionov, N. Kouprina, M. Eldarov, 1994

机译：通过链断断裂诱导分歧和同源DNA重复之间的转化相关的重组
8. Transformation-associated recombination between diverged and homologous DNA repeats is induced by strand breaks [R] . Larionov, V., Kouprina, N., Eldarov, M., 1994

机译：通过链断裂诱导分化和同源DNa重复之间的转化相关重组

Transformation-associated recombination between diverged and homologous DNA repeats is induced by strand breaks

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