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c-jun gene expression in human cells exposed to either ionizing radiation or hydrogen peroxide.

机译:暴露于电离辐射或过氧化氢的人细胞中的c-jun基因表达。

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We investigated the role of reactive oxygen intermediates (ROIs) and protein kinase C (PKC) in radiation- and H(sub 2)O(sub 2)-evoked c-jun gene expression in human HL-205 cells. This induction of c-jun gene expression could be prevented by pretreatment of the cells with Nacetylcysteine (an antioxidant) or H7 (a PKC and PKA inhibitor) but not by HA1004, a PKA inhibitor, suggesting a role for ROls and PKC in mediating c-jun gene expression. We also investigated potential differences in c-jun gene expression in a panel of normal and tumor cells untreated or treated with ionizing radiation or H(sub 2)O(sub 2). Treatment with radiation or H(sub 2)O(sub 2) produced a varied response, from some reduction to an increase of more than an order of magnitude in the steady-state level of c-jun mRNA. These data indicate that although induction of c-jun may be a common response to ionizing radiation and H(sub 2)O(sub 2), this response was reduced or absent in some cell types.

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