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Transfection of Cytochrome P450 cDNAs into Mammalian Cells Used in Mutation andTransformation Assays

机译：将细胞色素p450 cDNa转染到用于突变和转化分析的哺乳动物细胞中

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The present work demonstrates that cDNAs coding for cytochrome P450 enzymes canbe transfected into mammalian cells and expressed. Two different cell systems were used for transfection. The 10T 1/2 cells can be treated with a nontransforming dose of an initiator followed by continuous treatment with a second chemical that requires cytochrome P450 specific metabolism to manifest its promoting activity. By this approach, greater insight into the role of chemical metabolism in the promotion process (and presumably other nongenotoxic effects) can be obtained. Additionally, the role of specific cytochrome P450s in the metabolism of different classes of carcinogens/drugs can be elucidated. A major advantage of having the metabolizing enzymes actually present in the target cell is that effects of chemicals can be studied in long-term, low-dose exposure protocols which will eliminate the acute toxic effects which are associated with many current protocols.

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作者
Langenbach, R.; Crespi, C.; Davies, R.; Rudo, K.; Smith, P.;
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年度 1990
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总页数 12
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Deoxyribonucleic acids; Mutagens; Cells(Biology); Mammals; In vitro analysis; Carcinogens; Reprints;

机译：脱氧核糖核酸;诱变剂;细胞（生物学）;哺乳动物;体外分析;致癌物;重印;

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专利

1. TRANSFECTION OF SENDAI VIRUS F GENE CDNA WITH MUTATIONS AT ITS CLEAVAGE SITE AND HN GENE CDNA INTO COS CELLS INDUCES CELL FUSION [J] . Taira H, Sato T, Segawa H, Archives of virology . 1995,第1期

机译：将仙台病毒F基因CDNA在其切割位点进行突变转化，并将HN基因CDNA转化为COS细胞，从而诱导细胞融合
2. Characterization of diuron N-demethylation by mammalian hepatic microsomes and cDNA-expressed human cytochrome P450 enzymes. [J] . Abass K, Reponen P, Turpeinen M, Drug Metabolism and Disposition: The Biological Fate of Chemicals . 2007,第9期

机译：哺乳动物肝微粒体和cDNA表达的人细胞色素P450酶对diuron N-去甲基化的表征。
3. Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. [J] . Donato MT, Jimenez N, Castell JV, Drug Metabolism and Disposition: The Biological Fate of Chemicals . 2004,第7期

机译：用于筛选表达单个人P450酶的完整细胞中九种细胞色素P450（P450）活性的基于荧光的测定法。
4. Molecular Cloning and Sequence Analysis of Four New cDNA Fragments of Cytochrome P450 from Liposcelis bostrychophila Badonnel ( Psocoptera: Liposcelididae) [C] . Jiang Hongbo, Wang Jinjun, Xu Yongqiang, 第8届国际储藏物气调与熏蒸大会（The 8th International Conference on Controlled Atmosphere and Fumigation in Stored Products） . 2008

机译：博氏疏松脂肪酵母细胞色素P450的四个新cDNA片段的分子克隆和序列分析
5. Studies of the effects of single amino acid polymorphisms and mutations on the kinetic properties of cytochrome P450 1B1. [D] . Mammen, Jennifer Sophie. 2003

机译：研究单个氨基酸多态性和突变对细胞色素P450 1B1动力学特性的影响。
6. Coumarin Derivatives as Substrate Probes of Mammalian Cytochromes P450 2B4 and 2B6: Assessing the Importance of 7-Alkoxy Chain Length Halogen Substitution and Non-Active Site Mutations [O] . Jingbao Liu, Manish B. Shah, Qinghai Zhang, -1

机译：香豆素衍生物作为哺乳动物细胞色素P450 2B4和2B6的底物探针：评估7-烷氧基链长卤素取代和非活性位点突变的重要性。
7. Aromatase Cytochrome P450 in Rat Ovarian Granulosa Cells before and after Luteinization: Adenosine 3′,5′-Monophosphate-Dependent and Independent Regulation. Cloning and sequencing of rat aromatase cDNA and 5′ genomic DNA [O] . Gerard J. Hickey, Joel S. Krasnow, Wanda G. Beattie, 1990

机译：芳香酶细胞色素P450在大鼠卵巢颗粒细胞中，在丁黄嘌呤化之前和之后：腺苷3'，5'-单磷酸依赖性和独立的调节。大鼠芳香酶cDNA和5'基因组DNA的克隆和测序
8. Transfection of a Rat Cytochrome P450b cDNA into C3H10T1/2CL8 Mouse Embryo Fibroblasts [R] . Hansen, S. K., Ross, J. A., Siegried, J. M., 1989

机译：大鼠细胞色素p450b cDNa转染C3H10T1 / 2CL8小鼠胚胎成纤维细胞

Transfection of Cytochrome P450 cDNAs into Mammalian Cells Used in Mutation andTransformation Assays

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