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Development of Efficient and Stable Host-Vector Systems for Molecular Cloning in 'Bacillus subtilis'

机译：用于“枯草芽孢杆菌”分子克隆的高效稳定宿主载体系统的开发

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摘要

The development of efficient molecular cloning systems in the Gram-positive micro-organism Bacillus subtilis based on plasmid vectors derived from Staphylococcus aureus has met with several difficulties. Frequently observed problems with these plasmids were structural and segregational instability, and the low efficiencies in random cloning of foreign DNA (shotgun cloning). The latter is generally manifested in low yields of clones and limited insert sizes. The main purposes of the investigations described in the thesis were to uncover the major factors interfering with efficient molecular cloning in B. subtilis and, with this knowledge, to develop efficient and stable host-vector systems for this organism.

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作者
Haima, P.;
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年度 1989
页码 p.1-124
总页数 124
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Deoxyribonucleic acids ; Theses;

机译：脱氧核糖核酸;论文;

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专利

1. Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis. [J] . M Fujii, M Takagi, T Imanaka, Journal of bacteriology . 1983,第2期

机译：在载体质粒中从嗜热脂肪芽孢杆菌的热稳定中性蛋白酶基因的分子克隆及其在嗜热脂肪芽孢杆菌和枯草芽孢杆菌中的表达。
2. Purification and Characterization of Thermostable Pullulanase from Bacillus stearothermophilus and Molecular Cloning and Expression of the Gene in Bacillus subtilis [J] . Takashi Kuriki, Jong-Hyun Park, Shigetaka Okada, Applied and Environmental Microbiology . 1988,第11期

机译：芽孢杆菌枯草芽孢杆菌和枯草芽孢杆菌的分子克隆及基因表达的纯化与表征
3. Development of an intermolecular transposition assay system in Bacillus subtilis 168 using IS4Bsu1 from Bacillus subtilis (natto) [J] . Kiwamu Takahashi, Yasuhiko Sekine, Taku Chibazakura, Microbiology . 2007,第8期

机译：使用枯草芽孢杆菌（纳豆）（纳豆）的枯草芽孢杆菌168中芽孢杆菌168中的分子间转子测定系统的开发
4. Molecular Cloning and Biochemical Characterization of Oligo-1,6-Glucosidases from Bacillus subtilis and Bacillus licheniformis [C] . Xiaoming Hao, Ke Cai, Zixing Dong International conference on applied biotechnology . 2018

机译：枯草芽孢杆菌和地衣芽孢杆菌Oligo-1,6-葡糖苷酶的分子克隆和生化特性
5. Molecular cloning and characterization of a cluster of genes involved in the spore coat morphogenesis of Bacillus subtilis [D] . Zhang, Jianke 1993

机译：枯草芽孢杆菌芽孢形成形态相关基因簇的分子克隆与表征
6. Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis. [O] . M Fujii, M Takagi, T Imanaka, 1983

机译：在载体质粒中从嗜热脂肪芽孢杆菌的热稳定中性蛋白酶基因的分子克隆及其在嗜热脂肪芽孢杆菌和枯草芽孢杆菌中的表达。
7. Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis [O] . M Fujii, M Takagi, T Imanaka, 1983

机译：甲硝酸芽孢杆菌中芽孢杆菌中性蛋白酶基因的分子克隆及其在枯草芽孢杆菌和枯草芽孢杆菌中的表达
8. Cloning and Expression of the Bacillus anthracis Protective Antigen Gene in Bacillus subtilis. [R] . Ivins, B. E., Welkos, S. L. 1986

机译：枯草芽孢杆菌保护性抗原基因的克隆与表达。

Development of Efficient and Stable Host-Vector Systems for Molecular Cloning in 'Bacillus subtilis'

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