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Characterization and Nucleotide Sequence Determination of a Repeat Element Isolated from a 2,4,5-T Degrading Strain of 'Pseudomonas cepacia'

机译:从'pseudomonas cepacia'的2,4,5-T降解菌株中分离的重复元件的表征和核苷酸序列测定

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Pseudomonas cepacia strain AC1100, capable of growth on 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), was mutated to the 2,4,5-T(sup -) strain PT88 by a ColE1::Tn5 chromosomal insertion. Using cloned DNA from the region flanking the insertion, a 1477-bp sequence (designated RS1100) was identified which was repeated several times on the wild-type chromosome and was also present on AC1100 plasmid DNA. Various chromosomal fragments containing this sequence were cloned and their nucleotide sequence was determined. Examination of TS1100 revealed the presence of 38-39-bp terminal inverted repeats immediately flanked by 8-bp direct repeats. The translated sequence of the single large open reading frame of RS1100 showed structural similarity to the phage Mu transposes and other DNA-binding proteins. (Copyright (c) 1989 Elsevier Science Publishers B.V.)

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