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Isolation and Long-Term Culture of Rat, Rabbit, and Human Nasal Turbinate Epithelial Cells

机译：大鼠，兔和人鼻鼻甲上皮细胞的分离和长期培养

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Nasal turbinate epithelial cells were isolated from rats, rabbits, and humans using either a surgical or an in situ enzyme incubation technique. The culture conditions that permit optimal cell attachment and selective growth of the nasal epithelial cells were determined. These conditions will permit the long-term culture of these cells where typically 20 to 30 population doublings were observed. Differences between rat and human nasal epithelial cells were seen in substrate requirements, colony-forming efficiency, and response to fetal bovine serum and bovine serum albumin. These methodology and results will permit mechanistic studies of normal and abnormal cellular function and permit comparative response studies between nasal epithelial cells from rats and humans.

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作者
Steele, V. E. ; Arnold, J. T.;
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年度 1986
页码 1-10
总页数 10
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Cells(Biology); Rats; Rabbits; Humans; Isolation; Cultures(Biology); Reprints;

机译：细胞（生物）;大鼠;兔;人类;分离;培养物（生物学）;重印;

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1. Human Nasal Turbinates as a Viable Source of Respiratory Epithelial Cells Using Co-Culture System Versus Dispase Dissociation Technique. [J] . Noruddin NA, Saim AB, Chua KH, The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. . 2007,第12期

机译：使用共培养系统与分散酶解离技术，人鼻鼻甲是呼吸道上皮细胞的可行来源。
2. Changes of Gap and Tight Junctions during Differentiation of Human Nasal Epithelial Cells Using Primary Human Nasal Epithelial Cells and Primary Human Nasal Fibroblast Cells in a Noncontact Coculture System [J] . Jun-ichi Koizumi, Takashi Kojima, Ryuta Kamekura, The Journal of Membrane Biology . 2007,第1a3期

机译：非接触共培养系统中使用原代人鼻上皮细胞和原代人鼻成纤维细胞分化人鼻上皮细胞时间隙和紧密连接的变化
3. Isolation and culture of primary human nasal epithelial cells from anesthetized nasal epithelia [J] . HussainR., HugossonS., RoomansG.M. Acta Oto-Laryngologica . 2014,第3a4期

机译：从麻醉的鼻上皮细胞分离和培养原代人鼻上皮细胞
4. Co-culture of Mesenchymal Stem Cells and Respiratory Epithelial Cells to Engineer a Human Tracheal Mucosa [C] . Catherine Le Visage, Brian Dunham, Paul Flint, Annual meeting of the Society For Biomaterials . 2003

机译：间充质干细胞和呼吸道上皮细胞的共培养，以工程化人气管粘膜
5. Coculture of rabbit preimplantation embryos with rabbit oviduct epithelial cells. [D] . Carney, Edward Walter. 1990

机译：兔植入前胚胎与兔输卵管上皮细胞的共培养。
6. Patho-Biology of Nasal Polyps: 132 Characterization of 2 Epithelial Cell Air-Liquid Interface (ALI) Culture Models for Human Healthy Nasal Mucosa and Nasal Polyps [O] . Fco de Borja Callejas, Asunción Martínez-Antón, Jordi Roca-Ferrer, 2012

机译：鼻息肉的病理生物学：132人类健康的鼻黏膜和鼻息肉的两种上皮细胞气液界面（ALI）培养模型的特征
7. Human respiratory epithelial cells derived from the nasal turbinate express mesenchymal stem cell markers [O] . Aisha AM, Heikal MMY, Aminuddin BS, 2011

机译：源自鼻甲的人呼吸道上皮细胞表达间充质干细胞标志物
8. Respiratory Syncytial Virus Infection of Human Primary Nasal and Bronchial Epithelial Cell Cultures and Bronchoalveolar Macrophages. [R] . Becker, S., Soukup, J., Yankaskas, J. 1992

机译：呼吸道合胞病毒感染人原发性鼻和支气管上皮细胞培养及支气管肺泡巨噬细胞。

Isolation and Long-Term Culture of Rat, Rabbit, and Human Nasal Turbinate Epithelial Cells

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