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Evaluation of Surface Water Runoff from Swine Facilities for Contamination with Viruses

机译:猪病房污染地表径流的病虫害评价

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Molecular filtration was used to concentrate pseudorabies virus (PRV; Herpesvirus suis) from 6 liters of non-sterile water containing particulate matter from a natural source (creek or lake water) in which a known quantity of PRV was added. The molecular filtration technique used proved to have an average 63% recovery rate. Two surface water runoff samples were collected from each of 10 Nebraska swine farms where pseudorabies (PRV) virus infection and clinical disease had been diagnosed, and later confirmed by virus isolation or immunofluorescence. Each of the 20 samples were sonicated, clarified by low speed centrifugation and concentrated using the molecular filtration system. Aliquots of the concentrates were used to inoculate cell cultures for virus isolation.

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