• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国政府科技报告 >The Development of an Improved Method for the Detection of Enteric Viruses in Oysters.
【24h】

The Development of an Improved Method for the Detection of Enteric Viruses in Oysters.

机译：一种改进的牡蛎肠道病毒检测方法的研制。

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In order to assess the public health significance of enteric virus contamination of oysters,simple and reliable method are needed to detect enteric viruses in oyster meats. This report describes an improved method for detecting enteroviruses, reoviruses and adenoviruses in oyster tissue. In this method oyster meat is homogenized in chilled,sterile distilled water and adjusted to a pH of between 5.0and 5.5. The homogenate is centrifuged at low speed for 20min.,and the virus-free supernatant is discarded. The homogenate pellet is resuspended at an oyster solids-to-eluent ratio of 1/7in 0.05 M glycine-saline at pH 7.5and a conductivity of about 8000ppm NaC1for virus elution. The resuspended homogenate is centrifuged at low speed,and the virus-containing supernatant is prefiltered. The prefiltrate is condentrated to a volume of a few ml by ultrafiltration in a stirred ultrafiltration cell and inoculated directly into cell culture for virus assay. Using this method with 15to 60gram quantities of experimentally contaminated oyster meats,virus concentrations efficiencies averaged 47% for poliovirus, 44% for reovirus,and 47% for adenovirus,for an overall recovery efficiency of 46%.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号