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Phylogenetic Tree of 16S rRNA Sequences from Sulfate-Reducing Bacteria in a SandyMarine Sediment

机译：沙质海洋沉积物中硫酸盐还原菌16s rRNa序列的系统发育树

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Phylogenetic divergence among sulfate-reducing bateria in an estuarine sedimentsample was investigated by PCR amplification and comparison of partial 16S rDNA sequences. Twenty unique 16S rDNA sequences were found, 12 from delta subclass bacteria based on overall sequence similarity (82-91%). Two successive PCR amplifications were used to obtain and clone the 16S rDNA. The first reaction used templates derived from phosphate-buffered saline washed sediment with primers designed to amplify nearly full-length bacterial domain 16S rDNA. A produce from a first reaction was used as template in a second reaction with primers designed to selectivity amplify a region of 16S rDNA genes of sulfate-reducing bacteria. A phylogenetic tree incorporating the cloned sequences suggests the presence of yet to be cultivated lines of sulfate-reducing bacteria within the sediment sample.

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作者
Devereux, R.; Mundfrom, G. W.;
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年度 1994
页码 1-6
总页数 6
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Phylogeny; Sulfate-reducing bacteria; Marine biology; 16S ribosomal RNA; Sediments; Aquatic microbiology; Sequence alignment; Ribosomal DNA; Oligonucleotide probes; Reprints;

机译：系统发育;硫酸盐还原菌;海洋生物学; 16s核糖体RNa;沉积物;水生微生物学;序列比对;核糖体DNa;寡核苷酸探针;重印;

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专利

1. A phylogenetic tree of 16S rRNA sequences from sulfate-reducing bacteria in a sandy marine sediment. [J] . R Devereux, G W Mundfrom Applied and Environmental Microbiology . 1994,第9期

机译：沙质海洋沉积物中来自硫酸盐还原细菌的16S rRNA序列的系统树。
2. Monitoring the enrichment and isolation of sulfate-reducing bacteria by using oligonucleotide hybridization probes designed from environmentally derived 16S rRNA sequences. [J] . M D Kane, L K Poulsen, D A Stahl Applied and Environmental Microbiology . 1993,第3期

机译：通过使用由环境衍生的16S rRNA序列设计的寡核苷酸杂交探针来监测硫酸盐还原细菌的富集和分离。
3. The All-Species Living Tree project: A 16S rRNA-based phylogenetic tree of all sequenced type strains [J] . Yarza P, Richter M, Peplies J, Systematic and Applied Microbiology . 2008,第4期

机译：All-Species Living Tree项目：所有测序类型菌株的基于16S rRNA的系统树
4. Molecular phylogenetic relationships of different color forms within Harmonia axyridis Pallas (Coleoptera: Coccinellidae)based on sequences of 12S rRNA and 16S rRNA gene [C] . Dabin Yao, Defu Chi, Qingyu Wu, International conference on environmental biotechnology and materials engineering;EBME 2011 . 2011

机译：基于12S rRNA和16S rRNA基因序列的异色和谐虫内不同颜色形态的分子系统发育关系
5. The use of groEL and 16S rRNA gene sequences for classification of Ruminococcus albus and Ruminococcus flavefaciens bacterial species and for identification of unknown bacteria. [D] . Browne-Silva, Johnette Koffa. 2002

机译：groEL和16S rRNA基因序列在黄al球菌和黄褐球菌细菌种类分类和未知细菌鉴定中的应用。
6. A phylogenetic tree of 16S rRNA sequences from sulfate-reducing bacteria in a sandy marine sediment. [O] . R Devereux, G W Mundfrom 1994

机译：沙质海洋沉积物中来自硫酸盐还原细菌的16S rRNA序列的系统树。
7. Figure 2: Neighbor-joining phylogenetic tree of M. oxyfera-like bacterial 16S rRNA gene sequences in rhizosphere and non-rhizosphere sediments of P. crispus. [O] . -1

机译：图2：邻近M.的邻近邻近的M.Syiveria细菌16S rRNA基因序列在P. crispus的无菱形沉积物中。

Phylogenetic Tree of 16S rRNA Sequences from Sulfate-Reducing Bacteria in a SandyMarine Sediment

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