• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国政府科技报告 >Analytical Procedures and Quality Assurance Criteria for the Determination ofMajor and Minor Deoxynucleosides in Fish Tissue DNA by Liquid Chromatography-Ultraviolet Spectroscopy and Liquid Chromatography Thermospray Mass Spectrometry
【24h】

Analytical Procedures and Quality Assurance Criteria for the Determination ofMajor and Minor Deoxynucleosides in Fish Tissue DNA by Liquid Chromatography-Ultraviolet Spectroscopy and Liquid Chromatography Thermospray Mass Spectrometry

机译：液相色谱 - 紫外光谱和液相色谱热阵列质谱法测定鱼组织DNa中主要和次要脱氧核苷的分析方法和质量保证标准

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Analytical procedures and quality assurance criteria have been established forenzymatic hydrolysis of fish tissue DNA to free nucleosides and their subsequent characterization by liquid chromatography-photodiode-array ultraviolet spectroscopy and liquid chromatography-thermospray mass spectrometry. Optimization of enzymatic efficiency to assure minimal loss of modified nucleosides is described. Variability in analyte capacity factors and multiwavelength response have been compared for analyte standards and hydrolysates, and results have been used to derive qualitative and quantitative quality assurance criteria. A comparison of DNA mole percent calculated using single-wavelength quantification and multiwavelength averaging quantification indicates that less variability in data may be expected using the multiwavelength technique. Finally, the comparison of DNA from liver of three species of fish widely used in mutagen/carcinogen laboratory and field studies, rainbow trout (Onchorynchus mykiss), medaka (Oryzias latipes), and brown bullhead (Ictalurus nebulosus), has been determined. Identification of deoxyuridine in the DNA hydrolysates of each fish indicates that this analyte should be measured to accurately report DNA deoxynucleoside mole percent, especially when reporting data for the methylation of deoxycytidine. (Copyright (c) 1993 Elsevier Science Publishers B.V.)

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号