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Use of Perifusion to Evaluate Hormonal Release In vitro from Rat Pituitary andHypothalamic Tissue

机译:使用perifusion评估大鼠垂体和下丘脑组织的体外激素释放

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The use of in vitro procedures in reproductive toxicology has permitted a directassessment of hormonal release from isolated tissue and a means by which to determine potential sites of toxicant insult. The present chapter describes a perifusion procedure that can be used to evaluate stimulated or baseline hormonal secretion from pituitary and brain hypothalamic tissue. The implementation and utility of various combinations of tissue stimulation are presented, along with a discussion of the relative advantages and limitations of perifusion as an experimental approach.

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