首页> 美国政府科技报告 >Physiology and Molecular-Genetics of 'Thermus' and 'Bacillus'. 3. Plasmid Curing in 'Thermus' sp. E86264: Isolation and Partial Characterization of a Plasmid.

【24h】

Physiology and Molecular-Genetics of 'Thermus' and 'Bacillus'. 3. Plasmid Curing in 'Thermus' sp. E86264: Isolation and Partial Characterization of a Plasmid.

机译：'Thermus'和'Bacillus'的生理学和分子遗传学。 3.质粒在'Thermus'sp中固化。 E86264：质粒的分离和部分表征。

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Thermus sp. E86264 was cured of plasmids by the RNA polymerase inhibitor, acridine orange, and a plasmid was isolated and characterized. Plasmid profiles were analyzed by electrophoresis and a physical map of the plasmid was constructed using five site-specific endonucleases; Hind III, Pvu II, Nco I, Sal I and Dra III. Single restriction sites for Nco I, Sal I, and Dra III respectively were present. The size of the plasmid was 11kb, and the plasmid encoded for protease activity, and was associated with trimethoprim resistance. The size and the single restriction sites indicate that this plasmid, pTE264-1, may represent a useful cloning vector in Thermus and other thermophiles.

著录项

作者
Virtala, M. K.; Nordstroem, K. M.;
展开▼
作者单位

展开▼
年度 1992
页码 1-23
总页数 23
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Thermus; Plasmids; Restriction mapping; Electrophoresis; Acridine orange; Trimethoprim; Microbial drug resistance; Nucleic acid hybridization; Antibiotic resistance; Bacterial DNA; Foreign technology;

机译：Thermus;质粒;限制性作图;电泳;吖啶橙;甲氧苄啶;微生物抗药性;核酸杂交;抗生素抗性;细菌DNa;国外技术;

相似文献

外文文献
中文文献
专利

1. Plasmid curing in Thermus thermophilus and Thermus flavus [J] . Vaacute, squez Claudio, Vicuntilde, FEBS Letters . 1983,第2期

机译：嗜热栖热菌和黄热栖热菌的质粒固化
2. Sequence analysis and characterizations of two novel plasmids isolated from Thermus sp. 4C. [J] . Ruan L, Xu X Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems . 2007,第1期

机译：从Thermus sp。分离的两个新质粒的序列分析和鉴定。 4C。
3. The third plasmid pVV8 from Thermus thermophilus HB8: Isolation, characterization, and sequence determination [J] . Ohtani N., Tomita M., Itaya M. Extremophiles: Life under extreme conditions . 2012,第2期

机译：嗜热栖热菌HB8的第三个质粒pVV8：分离，鉴定和序列确定
4. Isolation and Characterization of Thermoalkalotolerant Bacillus sp. Strain ISTS2 for Carbon Dioxide Sequestration [C] . Smita Sundaram, Indu Shekhar Thakur International Humboldt Kolleg on Management of Water, Energy and Bio-Resources in the Era of Climate Change: Emerging issues and Challenges . 2014

机译：热蛋白醇杆菌SP的分离与表征。用于二氧化碳封存的菌株ISTS2
5. Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain [O] . Naoto Ohtani, Masaru Tomita, Mitsuhiro Itaya 2016

机译：固化嗜热栖热菌HB27的大质粒pTT27并在无质粒菌株中维持外源质粒
6. Plasmid curing in Thermus thermophilus and Thermus flavus [O] . Vásquez Claudio, Villanueva Jorge, Vicuña Rafael 1983

机译：嗜热栖热菌和黄热栖热菌的质粒固化
7. Physiology and Molecular-Genetics of 'Thermus' and 'Bacillus'. 5. Biochemistryand Physiology of Wild Type and Plasmid-Cured 'Thermus' Strains [R] . Nordstroem, K. M., Virtala, M. K. 1993

机译：'Thermus'和'Bacillus'的生理学和分子遗传学。 5.野生型和质粒固化'Thermus'菌株的生物化学和生理学

Physiology and Molecular-Genetics of 'Thermus' and 'Bacillus'. 3. Plasmid Curing in 'Thermus' sp. E86264: Isolation and Partial Characterization of a Plasmid.

摘要

著录项

相似文献

相关主题

期刊订阅