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Sensitive protein detection using an optical fibre long period grating sensor anchored with silica core gold shell nanoparticles

机译:使用锚定有硅核金壳纳米粒子的光纤长周期光栅传感器进行敏感蛋白检测

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摘要

An optical fibre long period grating (LPG), modified with a coating of silica gold (SiO2:Au) core/shell nanoparticles (NPs) deposited using the layer-by-layer (LbL) method, was employed for the development of a bio-sensor. The SiO2:Au NPs were electrostatically assembled onto the LPG with the aid of a poly(hydrochloride ammonium) (PAH) polycation layer. The LPG sensor operates at the phase matching turning point to provide the highest sensitivity. The SiO2:Au NPs were modified with biotin, which was used as a ligand for streptavidin (SV) detection. The sensing mechanism is based on the measurement of the refractive index change induced by the binding of the SV to the biotin. The lowest detected concentration of SV was 19 nM using an LPG modified with a 3 layer (PAH/SiO2:Au) thin film.
机译:使用通过逐层(LbL)方法沉积的二氧化硅金(SiO2:Au)核/壳纳米颗粒(NPs)涂层修饰的光纤长周期光栅(LPG)用于生物材料的开发-传感器。借助于聚(氯化铵)(PAH)聚阳离子层将SiO2:Au NP静电组装到LPG上。 LPG传感器在相位匹配转折点工作,以提供最高的灵敏度。用生物素修饰了SiO2:Au NP,用作链霉亲和素(SV)检测的配体。感测机制基于对SV与生物素结合引起的折射率变化的测量。使用经3层(PAH / SiO2:Au)薄膜改性的LPG,SV的最低检测浓度为19 nM。

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