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Saprolegnia Pathogen from Pengze Crucian Carp (Carassius auratus var. Pengze) Eggs and its Control with Traditional Chinese Herb

机译：彭泽鲫鱼（Carassius Auratus var。彭泽）鸡蛋及其与中文草本植物的控制等Saprongnia病原体

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In the present study, a pathogenic strain, JL, was isolated from Pengze crucian carp (Carassius auratus) eggs suffering from saprolegniosis. It was initially determined as Saprolegnia sp. strain JL. Saprolegnia species have been implicated for significant fungal contamination, involving both living and dead fish and their eggs. A phylogenetic tree was constructed using the maximum parsimony method. The tree shows that the JL strain was closely related to Saprolegnia parasitica isolate SAP171, isolated from Salmo trutta suffering from saprolegniasis in Laukaa, Finland. The minimum inhibitory concentration (MIC) of 20 Chinese herbs was screened. Pseudolarix kaempferi Gord. (Pinaceae) was the most effective in inhibiting growth of the bacteria and was chosen for further trial. Significant protective efficacy of 52.63% and 73.68% was obtained against the JL strain in Pengze crucian carp eggs at P. kaempferi concentrations of 12.5 mg/ml and 25.0 mg/ml, respectively.
机译：在本研究中，从患腐烂病的彭泽cru鱼（Carassius auratus）卵中分离出一种病原菌株JL。最初被确定为腐霉菌。菌株JL。腐霉菌物种被认为与真菌的污染有关，包括活鱼和死鱼及其卵。使用最大简约方法构建了系统发育树。该树表明，该JL菌株与分离自Saprolegnia parasitica的SAP171分离株SAP171密切相关，SAP171分离自芬兰劳卡州患有腐烂病的Salmo trutta。筛选了20种中草药的最小抑菌浓度（MIC）。 Pseudolarix kaempferi Gord。 （木犀科）在抑制细菌生长方面最有效，因此被选作进一步试验。在彭氏ka鱼卵中，分别以12.5 mg / ml和25.0 mg / ml的浓度对彭泽cru鱼卵中的JL菌株获得了52.63％和73.68％的显着保护作用。

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