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首页> 外文OA文献 >A Polysaccharide Purified from Morchella conica Pers. Prevents Oxidative Stress Induced by H2O2 in Human Embryonic Kidney (HEK) 293T Cells
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A Polysaccharide Purified from Morchella conica Pers. Prevents Oxidative Stress Induced by H2O2 in Human Embryonic Kidney (HEK) 293T Cells

机译：从莫尔卡拉Conica纯化的多糖。防止H2O2在人胚胎肾（HEK）293T细胞中诱导的氧化应激

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Morchella conica Pers. (M. conica) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from M. conica have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral M. conica polysaccharides-2) purified from M. conica, as determined by radical scavenging assay and H2O2-induced oxidative stress in HEK 293T cells. Results showed that NMCP-2 with an average molecular weight of 48.3 kDa possessed a much stronger chelating ability on ferrous ions and a higher ability to scavenge radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) than the other purified fraction of NMCP-1 from M. conica. Moreover, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zolium bromide (MTT) assay showed that NMCP-2 dose-dependently preserved cell viability of H2O2-induced cells. The NMCP-2 pretreated group reduced the generation of reactive oxygen species (ROS) content and increased the mitochondria membrane potential (MMP) levels. In addition, Hoechst 33342 staining revealed cells treated with NMCP-2 declined nuclear condensation. Ultrastructural observation revealed that NMCP-2 pretreatment alleviated the ruptured mitochondria when exposed to H2O2. Furthermore, western blot analysis showed that NMCP-2 prevented significant downregulation of the protein expression of Bax, cleaved caspases 3, and upregulated Bcl-2 levels. These results suggest the protective effects of NMCP-2 against H2O2-induced injury in HEK 293T cells. NMCP-2 could be used as a natural antioxidant of functional foods and natural drugs.
机译：尖顶羊肚菌个人（M. conica）已被同时用作医疗和食用菌和具有抗微生物性能和抗氧化活性。然而，从M.纯化多糖的抗氧化性能conica还没有被研究。本研究的目的是调查的多糖的体外抗氧化性能NMCP-2（中性M. conica多糖-2）来自结核分枝conica纯化，如通过在HEK 293T细胞中的自由基清除测定和过氧化氢诱导的氧化应激来确定。结果表明，NMCP-2与48.3千道尔顿的平均分子量大于具有NMCP的其他纯化级分上的亚铁离子强得多的螯合能力和以清除自由基清除2,2-二苯基-1-苦基苯肼（DPPH）更高的能力从-1 M. conica。此外，3-（4,5二甲基噻唑-2-基）-2，5- diphenyltetra-zolium溴化物（MTT）测定法表明，NMCP-2剂量依赖性地保存过氧化氢诱导的细胞的细胞生存力。所述NMCP-2预处理组减少的反应性氧物质（ROS）的内容的产生和增加的线粒体膜电位（MMP）的水平。此外，赫斯特33342染色显示与NMCP-2处理的细胞减少核固缩。电镜观察发现，当暴露于H 2 O 2 NMCP-2预处理减轻了破裂的线粒体。此外，免疫印迹分析表明，NMCP-2防止Bax蛋白的蛋白质表达的下调显著，切割胱天蛋白酶3，和上调Bcl-2水平。这些结果表明NMCP-2对在HEK 293T细胞中过氧化氢诱导损伤的保护作用。 NMCP-2可作为功能性食品和天然药物天然抗氧化剂。

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